BRENDA - Enzyme Database
show all sequences of 2.7.1.81

The partial purification and properties of hydroxylysine kinase from rat liver

Hiles, R.A.; Henderson, LaVell M.; J. Biol. Chem. 247, 646-651 (1972)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-Amino-5-hydroxyadipate
4 isomers, 40 mM, strong inhibition
Rattus norvegicus
4,5-Dehydro-(trans)-L-lysine
-
Rattus norvegicus
carnitine
2 isomers
Rattus norvegicus
choline
40 mM, weak inhibition
Rattus norvegicus
D-Lysine
-
Rattus norvegicus
D-ornithine
-
Rattus norvegicus
ethanolamine
40 mM, weak inhibition
Rattus norvegicus
Hydroxy-L-pipecolate
-
Rattus norvegicus
hydroxy-L-proline
; 40 mM, strong
Rattus norvegicus
L-2,4-diamino-n-butyrate
-
Rattus norvegicus
L-homoserine
40 mM, weak inhibition
Rattus norvegicus
L-lysine
higher inhibitory effect than with D-lysine
Rattus norvegicus
L-ornithine
higher inhibitory effect than with D-ornithine
Rattus norvegicus
Mn2+
at 1 mM, in presence of 1 mM Mg2+, strong inhibition, but activation in absence of Mg2+
Rattus norvegicus
S-aminoethyl-L-cysteine
-
Rattus norvegicus
Zn2+
at 1 mM, in presence of 1 mM Mg2+, strong inhibition
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0056
-
hydroxy-L-lysine
pH 8.0, 37°C
Rattus norvegicus
0.0062
-
allohydroxy-L-lysine
pH 8.0, 37°C
Rattus norvegicus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+
Rattus norvegicus
Co2+
requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+
Rattus norvegicus
Cu2+
requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+
Rattus norvegicus
Fe2+
requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+
Rattus norvegicus
Hg2+
requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+
Rattus norvegicus
Mg2+
requirement of a divalent metal ion, Mg2+ yields highest activity
Rattus norvegicus
Mn2+
requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+, strong inhibition in presence of 1 mM Mg2+
Rattus norvegicus
additional information
K+, Li+ have no influence
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
GTP + 5-hydroxy-L-lysine
Rattus norvegicus
erythro-5-hydroxy-L8-lysine
GDP + O-phosphohydroxy-L-lysine
-
Rattus norvegicus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Cebus albifrons
-
-
-
Chlorocebus aethiops
-
-
-
Gallus gallus
-
-
-
Mus musculus
-
-
-
no activity in Homo sapiens
-
no activity in liver and kidney
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
1200fold; partial
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Cebus albifrons
-
kidney
-
Chlorocebus aethiops
-
kidney
-
Mus musculus
-
kidney
-
Oryctolagus cuniculus
-
kidney
-
Rattus norvegicus
-
liver
-
Bos taurus
-
liver
-
Cebus albifrons
-
liver
-
Gallus gallus
-
liver
-
Mus musculus
-
liver
-
Oryctolagus cuniculus
-
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.114
-
-
Rattus norvegicus
Storage Stability
Storage Stability
Organism
frozen, little loss of activity for weeks
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GTP + 5-hydroxy-L-lysine
erythro-5-hydroxy-L8-lysine
642034
Rattus norvegicus
GDP + O-phosphohydroxy-L-lysine
-
642034
Rattus norvegicus
?
GTP + allohydroxy-L-lysine
-
642034
Rattus norvegicus
?
-
-
-
?
additional information
no activity with D-isomers, ITP can partially substitute for GTP
642034
Rattus norvegicus
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
-
-
Rattus norvegicus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9
25% of maximal activity at pH 6.0, 35% of maximal activity at pH 9.0
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-Amino-5-hydroxyadipate
4 isomers, 40 mM, strong inhibition
Rattus norvegicus
4,5-Dehydro-(trans)-L-lysine
-
Rattus norvegicus
carnitine
2 isomers
Rattus norvegicus
choline
40 mM, weak inhibition
Rattus norvegicus
D-Lysine
-
Rattus norvegicus
D-ornithine
-
Rattus norvegicus
ethanolamine
40 mM, weak inhibition
Rattus norvegicus
Hydroxy-L-pipecolate
-
Rattus norvegicus
hydroxy-L-proline
; 40 mM, strong
Rattus norvegicus
L-2,4-diamino-n-butyrate
-
Rattus norvegicus
L-homoserine
40 mM, weak inhibition
Rattus norvegicus
L-lysine
higher inhibitory effect than with D-lysine
Rattus norvegicus
L-ornithine
higher inhibitory effect than with D-ornithine
Rattus norvegicus
Mn2+
at 1 mM, in presence of 1 mM Mg2+, strong inhibition, but activation in absence of Mg2+
Rattus norvegicus
S-aminoethyl-L-cysteine
-
Rattus norvegicus
Zn2+
at 1 mM, in presence of 1 mM Mg2+, strong inhibition
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0056
-
hydroxy-L-lysine
pH 8.0, 37°C
Rattus norvegicus
0.0062
-
allohydroxy-L-lysine
pH 8.0, 37°C
Rattus norvegicus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+
Rattus norvegicus
Co2+
requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+
Rattus norvegicus
Cu2+
requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+
Rattus norvegicus
Fe2+
requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+
Rattus norvegicus
Hg2+
requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+
Rattus norvegicus
Mg2+
requirement of a divalent metal ion, Mg2+ yields highest activity
Rattus norvegicus
Mn2+
requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+, strong inhibition in presence of 1 mM Mg2+
Rattus norvegicus
additional information
K+, Li+ have no influence
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
GTP + 5-hydroxy-L-lysine
Rattus norvegicus
erythro-5-hydroxy-L8-lysine
GDP + O-phosphohydroxy-L-lysine
-
Rattus norvegicus
?
Purification (Commentary) (protein specific)
Commentary
Organism
1200fold; partial
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Cebus albifrons
-
kidney
-
Chlorocebus aethiops
-
kidney
-
Mus musculus
-
kidney
-
Oryctolagus cuniculus
-
kidney
-
Rattus norvegicus
-
liver
-
Bos taurus
-
liver
-
Cebus albifrons
-
liver
-
Gallus gallus
-
liver
-
Mus musculus
-
liver
-
Oryctolagus cuniculus
-
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.114
-
-
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
frozen, little loss of activity for weeks
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GTP + 5-hydroxy-L-lysine
erythro-5-hydroxy-L8-lysine
642034
Rattus norvegicus
GDP + O-phosphohydroxy-L-lysine
-
642034
Rattus norvegicus
?
GTP + allohydroxy-L-lysine
-
642034
Rattus norvegicus
?
-
-
-
?
additional information
no activity with D-isomers, ITP can partially substitute for GTP
642034
Rattus norvegicus
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
-
-
Rattus norvegicus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9
25% of maximal activity at pH 6.0, 35% of maximal activity at pH 9.0
Rattus norvegicus
Other publictions for EC 2.7.1.81
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
720057
Veiga-da-Cunha
Molecular identification of hy ...
Homo sapiens
J. Biol. Chem.
287
7246-7255
2012
-
-
1
-
-
-
-
4
-
-
-
-
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
4
4
642035
Chang
Guanosine triphosphate: 5-hydr ...
Rattus norvegicus
Enzyme
22
230-234
1977
-
-
-
-
-
-
7
1
-
1
-
1
-
1
-
-
1
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
1
-
1
-
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
642034
Hiles
The partial purification and p ...
Bos taurus, Cebus albifrons, Chlorocebus aethiops, Gallus gallus, Mus musculus, no activity in Homo sapiens, Oryctolagus cuniculus, Rattus norvegicus
J. Biol. Chem.
247
646-651
1972
-
-
-
-
-
-
16
2
-
8
-
1
-
9
-
-
1
-
-
12
1
1
3
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
16
-
2
-
8
-
1
-
-
-
1
-
12
1
1
3
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-