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Literature summary for 2.7.1.80 extracted from
- Enzymatic phosphorylation of serine (1972), J. Biol. Chem., 247, 3382-3392.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 50% inhibition at 1 mM | Propionibacterium freudenreichii subsp. shermanii |  |
| Cd2+ | 65% inhibition at 1 mM | Propionibacterium freudenreichii subsp. shermanii | |
| D-serine | 20 mM, weak inhibition | Propionibacterium freudenreichii subsp. shermanii | |
| DL-homoserine | 100 mM, weak inhibition | Propionibacterium freudenreichii subsp. shermanii | |
| EDTA | inpresence of phosphate | Propionibacterium freudenreichii subsp. shermanii | |
| glycerol | 100 mM, weak inhibition | Propionibacterium freudenreichii subsp. shermanii | |
| glycine | 20% inhibition at 10 mM | Propionibacterium freudenreichii subsp. shermanii | |
| Glycyl-L-serine | weak inhibition | Propionibacterium freudenreichii subsp. shermanii | |
| Hg2+ | 55% inhibition at 1 mM | Propionibacterium freudenreichii subsp. shermanii | |
| L-alanine | 35% inhibition at 10 mM | Propionibacterium freudenreichii subsp. shermanii | |
| L-threonine | 100 mM, weak inhibition | Propionibacterium freudenreichii subsp. shermanii | |
| La3+ | 25% inhibition at 1 mM | Propionibacterium freudenreichii subsp. shermanii | |
| phosphate | competitive | Propionibacterium freudenreichii subsp. shermanii | |
| Sr2+ | weak inhibition | Propionibacterium freudenreichii subsp. shermanii | |
| Tris | 0.6 M, weak inhibition | Propionibacterium freudenreichii subsp. shermanii | |
| Zn2+ | 85% inhibition at 10 mM | Propionibacterium freudenreichii subsp. shermanii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
diphosphate | pH 7.8, 37°C | Propionibacterium freudenreichii subsp. shermanii | |
| 0.13 | - |
Mg2+ | 1 mM diphosphate, pH 7.8, 37°C | Propionibacterium freudenreichii subsp. shermanii | |
| 1.9 | - |
L-serine | pH 7.8, 37°C | Propionibacterium freudenreichii subsp. shermanii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | slight stimulation, about 14% of activity compared to Mg2+ | Propionibacterium freudenreichii subsp. shermanii | |
| Co2+ | slight stimulation, about 42% of activity compared to Mg2+ | Propionibacterium freudenreichii subsp. shermanii | |
| Cu2+ | slight stimulation, about 14% of activity compared to Mg2+ | Propionibacterium freudenreichii subsp. shermanii | |
| Mg2+ | high stimulation | Propionibacterium freudenreichii subsp. shermanii | |
| Mn2+ | slight stimulation, about 34% of activity compared to Mg2+ | Propionibacterium freudenreichii subsp. shermanii | |
| Zn2+ | slight stimulation, about 14% of activity compared to Mg2+ | Propionibacterium freudenreichii subsp. shermanii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
gel filtration | Propionibacterium freudenreichii subsp. shermanii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + L-serine | Propionibacterium freudenreichii subsp. shermanii | - |
phosphate + O-phospho-L-serine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Propionibacterium freudenreichii subsp. shermanii | - |
strain ATCC 9614 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, 102fold | Propionibacterium freudenreichii subsp. shermanii |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| diphosphate + L-serine = phosphate + O-phospho-L-serine | binary or ping-pong mechanism | Propionibacterium freudenreichii subsp. shermanii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
partially purified enzyme | Propionibacterium freudenreichii subsp. shermanii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C o r-15°C, 0.02 M Tris buffer, pH 7.8, stable for several weeks | Propionibacterium freudenreichii subsp. shermanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + alpha-methylserine | 7% activity compared to L-serine | Propionibacterium freudenreichii subsp. shermanii | phosphate + alpha-methyl-O-phospho-L-serine | - |
? | |
| diphosphate + DL-homoserine | 7% activity compared to L-serine | Propionibacterium freudenreichii subsp. shermanii | phosphate + O-phospho-DL-homoserine | - |
? | |
| diphosphate + glycyl-L-serine | 16% activity compared to L-serine | Propionibacterium freudenreichii subsp. shermanii | phosphate + glycyl-O-phospho-L-serine | - |
? | |
| diphosphate + L-serine | - |
Propionibacterium freudenreichii subsp. shermanii | phosphate + O-phospho-L-serine | - |
? | |
| diphosphate + L-threonine | 8% activity compared to L-serine | Propionibacterium freudenreichii subsp. shermanii | phosphate + O-phospho-L-threonine | - |
? | |
| diphosphate + N-chloroacetyl-L-serine | 19% activity compared to L-serine | Propionibacterium freudenreichii subsp. shermanii | phosphate + N-chloroacetyl-O-phospho-L-serine | - |
? | |
| additional information | substrate specificity | Propionibacterium freudenreichii subsp. shermanii | ? | - |
? | |
| additional information | no activity with D-serine and L-cysteine, tripolyphosphate can substitute to some extent for diphosphate | Propionibacterium freudenreichii subsp. shermanii | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Propionibacterium freudenreichii subsp. shermanii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
stable at least 12 h | Propionibacterium freudenreichii subsp. shermanii |
| 37 | - |
stable at least 1 h | Propionibacterium freudenreichii subsp. shermanii |
| 65 | - |
15 min, 46% remaining activity | Propionibacterium freudenreichii subsp. shermanii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Propionibacterium freudenreichii subsp. shermanii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | 7.8 | 60% of maximal activity at pH 5.4 and pH 7.8 | Propionibacterium freudenreichii subsp. shermanii |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
glycine | pH 7.8, 37°C | Propionibacterium freudenreichii subsp. shermanii | |
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Release 2023.1 (January 2023)
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