Cloned (Comment) | Organism |
---|---|
- |
Vibrio cholerae serotype O1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
D-glucosamine | pH 7.0, 25°C | Vibrio cholerae serotype O1 | |
1.96 | - |
ATP | pH 7.0, 25°C | Vibrio cholerae serotype O1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Km: 1.98 mM | Vibrio cholerae serotype O1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE | Vibrio cholerae serotype O1 |
31600 | - |
x * 31600, calculation from nucleotide sequence | Vibrio cholerae serotype O1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae serotype O1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Vibrio cholerae serotype O1 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
92.5 | - |
- |
Vibrio cholerae serotype O1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucosamine | enzyme is specific for D-glucosamine | Vibrio cholerae serotype O1 | ADP + D-glucosamine 6-phosphate | - |
? | |
GTP + D-glucosamine | 10% of the activity with ATP | Vibrio cholerae serotype O1 | GDP + D-glucosamine 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Vibrio cholerae serotype O1 |
? | x * 31600, calculation from nucleotide sequence | Vibrio cholerae serotype O1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 42 | - |
Vibrio cholerae serotype O1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 30 | 30 min, stable | Vibrio cholerae serotype O1 |
37 | - |
30 min, retains at least 91% of its activity | Vibrio cholerae serotype O1 |
65 | - |
30 min, complete loss of activity | Vibrio cholerae serotype O1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Vibrio cholerae serotype O1 |