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Literature summary for 2.7.1.78 extracted from

  • Munir, A.; Shuman, S.
    Characterization of Runella slithyformis HD-Pnk, a bifunctional DNA/RNA end-healing enzyme composed of an N-terminal 2,3-phosphoesterase HD domain and a C-terminal 5'-OH polynucleotide kinase domain (2017), J. Bacteriol., 199, e00739-16 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.78

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Runella slithyformis

Protein Variants

Protein Variants Comment Organism
D254A inactive Runella slithyformis
H73A the mutant shows wild type activity Runella slithyformis
R257A inactive Runella slithyformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.104
-
 ATP at pH 8.0 and 37°C Runella slithyformis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Mg2+, 5 mM used in assay conditions Runella slithyformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5'-dephospho-DNA Runella slithyformis
-
 ADP + 5'-phospho-DNA
-
 ?
ATP + 5'-dephospho-DNA Runella slithyformis ATCC 49304
-
 ADP + 5'-phospho-DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Runella slithyformis
-
-
-
Runella slithyformis ATCC 49304
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His60 Ni Superflow resin column chromatography Runella slithyformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5'-CTAGAGCTACAATTGCGACCG-3'
-
 Runella slithyformis ADP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3'
-
 ?
ATP + 5'-CTAGAGCTACAATTGCGACCG-3'
-
 Runella slithyformis ATCC 49304 ADP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3'
-
 ?
ATP + 5'-dephospho-DNA
-
 Runella slithyformis ADP + 5'-phospho-DNA
-
 ?
ATP + 5'-dephospho-DNA
-
 Runella slithyformis ATCC 49304 ADP + 5'-phospho-DNA
-
 ?
CTP + 5'-CTAGAGCTACAATTGCGACCG-3'
-
 Runella slithyformis CDP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3'
-
 ?
CTP + 5'-CTAGAGCTACAATTGCGACCG-3'
-
 Runella slithyformis ATCC 49304 CDP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3'
-
 ?
dATP + 5'-CTAGAGCTACAATTGCGACCG-3'
-
 Runella slithyformis dADP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3'
-
 ?
dATP + 5'-CTAGAGCTACAATTGCGACCG-3'
-
 Runella slithyformis ATCC 49304 dADP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3'
-
 ?
GTP + 5'-CTAGAGCTACAATTGCGACCG-3'
-
 Runella slithyformis GDP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3'
-
 ?
additional information the enzyme also has RNA 2',3-cyclic phosphodiesterase, DNA 3'-phosphatase and RNA 2'- and 3'-phosphomonoesterase activities Runella slithyformis ?
-
-
additional information the enzyme also has RNA 2',3-cyclic phosphodiesterase, DNA 3'-phosphatase and RNA 2'- and 3'-phosphomonoesterase activities Runella slithyformis ATCC 49304 ?
-
-
UTP + 5'-CTAGAGCTACAATTGCGACCG-3'
-
 Runella slithyformis UDP + 5'-phospho-CTAGAGCTACAATTGCGACCG-3'
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Runella slithyformis

Synonyms

Synonyms Comment Organism
5'-OH polynucleotide kinase
-
 Runella slithyformis
HD-Pnk the enzyme consists of an N-terminal 2',3'-phosphoesterase module of the HD domain family fused to a C-terminal polynucleotide kinase module Runella slithyformis
polynucleotide kinase
-
 Runella slithyformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.183
-
 ATP at pH 6.0 and 37°C Runella slithyformis