Literature summary for 2.7.1.78 extracted from

Dong, Z.; Zhang, L.; Qiao, M.; Ge, J.; Liu, A.; Li, Z.
A label-free assay for T4 polynucleotide kinase/phosphatase activity and its inhibitors based on poly(thymine)-templated copper nanoparticles (2015), Talanta, 146, 253-258 .

Search for more literature for 2.7.1.78

Application

Application	Comment	Organism
analysis	label-free fluorescent assay for T4 polynucleotidekinase/phosphatase PNKP activity using poly(thymine)-templated fluorescent copper nanoparticles as a fluorescent indicator. A T-rich hairpin primer with a 3'-phosphoryl end, serves as both the substrate for T4 PNKP and DNA template for the formation of fluorescent copper nanoparticles. Upon hydrolysis by T4 PNKP, the resulting hairpin primer with a 3'-hydroxyl end is elongated to form a long double-strand product by DNA polymerase, which prohibits the formation of fluorescent copper nanoparticles	Tequatrovirus T4

Organism

Organism	UniProt	Comment	Textmining
Tequatrovirus T4	P06855	-	-

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)