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Literature summary for 2.7.1.78 extracted from

  • Zhang, X.; Liu, Q.; Jin, Y.; Li, B.
    Determination of the activity of T4 polynucleotide kinase phosphatase by exploiting the sequence-dependent fluorescence of DNA-templated copper nanoclusters (2019), Microchim. Acta, 186, 003 .
    View publication on PubMed

Application

Application Comment Organism
analysis fluorometric method for the determination of the activity of T4 polynucleotide kinase phosphatase PNKP. A partially doublestranded DNA substrate is dephosphorylated on addition of T4 PNKP to generate the long dsDNA, which acts as a template for synthesizing copper nanoclusters. The assay has an analytical range from 0.07 U/ml to 15 U/ml and a detection limit of 0.06 U/ml Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4 P06855
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