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Literature summary for 2.7.1.78 extracted from

  • Whiteside, J.R.; Box, C.L.; McMillan, T.J.; Allinson, S.L.
    Cadmium and copper inhibit both DNA repair activities of polynucleotide kinase (2010), DNA Repair, 9, 83-89.
    View publication on PubMed
Search for more literature for 2.7.1.78

Cloned(Commentary)

Cloned (Comment) Organism
a vector encoding N-terminally Strep II epitope-tagged polynucleotide kinase is prepared, the protein is expressed in Escherichia coli Rosetta 2 DE3 pLysS cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
 Homo sapiens
Cu2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5'-dephospho-DNA Homo sapiens
-
 ADP + 5'-phospho-DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Strep-Tactin resin Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
MRC-5 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5'-dephospho-DNA
-
 Homo sapiens ADP + 5'-phospho-DNA
-
 ?

Synonyms

Synonyms Comment Organism
PNK
-
 Homo sapiens
polynucleotide kinase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 inhibition assay Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 inhibition assay Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens