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Literature summary for 2.7.1.78 extracted from

  • Zhu, H.; Yin, S.; Shuman, S.
    Characterization of polynucleotide kinase/phosphatase enzymes from Mycobacteriophages omega and Cjw1 and vibriophage KVP40 (2004), J. Biol. Chem., 279, 26358-26369.
    View publication on PubMed
Search for more literature for 2.7.1.78

Protein Variants

Protein Variants Comment Organism
D169A 50% of wild-type activity with 37-mer oligodeoxyribonucleotide substrate, no reverse reaction Kostyavirus CJW1
D175A 60% of wild-type activity with 37-mer oligodeoxyribonucleotide substrate, 68% of wild-type activity with CMP substrate Omegavirus omega
K15A no enzymic activity in kinase reaction, 58% of wild-type activity in 3’-phosphates reaction Kostyavirus CJW1
K16A no enzymic activity Omegavirus omega

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ 1-2 mM, maximum activity, inhibitory above Kostyavirus CJW1
Mg2+ 1-2 mM, maximum activity, inhibitory above Omegavirus omega

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 ATP pH 6.5, 37°C Omegavirus omega
0.04
-
 ATP pH 6.5, 37°C Kostyavirus CJW1

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ maximum activity at 0.6-2.5 mM Kostyavirus CJW1
Mg2+ 1-2 mM, maximum activity, inhibitory above Omegavirus omega
Mg2+ about 60% as efficient as Co2+ Kostyavirus CJW1
Mn2+ half as effective as Mg2+ Omegavirus omega
Mn2+ about 60% as efficient as Co2+ Kostyavirus CJW1
additional information divalent metal ion required Omegavirus omega

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 4 * 39000, recombinant protein with His10-tag, calculated, protein is either a stable but asymmetrically shaped homotetramer or a metastable tetramer that is prone to dissociate to a homodimer Kostyavirus CJW1
40000
-
 4 * 40000, recombinant protein with His10-tag, SDS-PAGE, protein is either a stable but asymmetrically shaped homotetramer or a metastable tetramer that is prone to dissociate to a homodimer Omegavirus omega
80000 160000 sedimentation equilibrium analysis Omegavirus omega
80000 160000 sedimentaion equilibrium analysis Kostyavirus CJW1

Organism

Organism UniProt Comment Textmining
Kostyavirus CJW1
-
 expression in Escherichia coli with His10-tag
-
Omegavirus omega
-
 expression in Escherichia coli with His10-tag
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3'-CMP
-
 Omegavirus omega ADP + pCp
-
 ?
ATP + 3'-CMP
-
 Kostyavirus CJW1 ADP + pCp
-
 ?
ATP + 5'-dephospho-DNA 37-mer oligodeoxyribonucleotide substrate Omegavirus omega ADP + 5'-phospho-DNA
-
 r
ATP + 5'-dephospho-DNA 37-mer oligodeoxyribonucleotide substrate Kostyavirus CJW1 ADP + 5'-phospho-DNA
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 39000, recombinant protein with His10-tag, calculated, protein is either a stable but asymmetrically shaped homotetramer or a metastable tetramer that is prone to dissociate to a homodimer Kostyavirus CJW1
tetramer 4 * 40000, recombinant protein with His10-tag, SDS-PAGE, protein is either a stable but asymmetrically shaped homotetramer or a metastable tetramer that is prone to dissociate to a homodimer Omegavirus omega

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0043
-
 ATP pH 6.5, 37°C Kostyavirus CJW1
0.008
-
 3'-CMP pH 6.5, 37°C Kostyavirus CJW1
0.2
-
 ATP pH 6.5, 37°C Omegavirus omega
1.6
-
 3'-CMP pH 6.5, 37°C Omegavirus omega

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7.5 3’-CMP kinase activit Kostyavirus CJW1
8.5 9.5
-
 Omegavirus omega

pH Range

pH Minimum pH Maximum Comment Organism
6
-
 50% of maximum activity Omegavirus omega
6
-
 50% of maximum activity Kostyavirus CJW1