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Literature summary for 2.7.1.78 extracted from

  • Shuman, S.; Hurwitz, J.
    5-Hydroxyl polyribonucleotide kinase from HeLa cell nuclei. Purification and properties (1979), J. Biol. Chem., 254, 10396-10404.
    View publication on PubMed
Search for more literature for 2.7.1.78

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin
-
 Homo sapiens
dithiothreitol
-
 Homo sapiens
sulfhydryl reagents
-
 Homo sapiens

General Stability

General Stability Organism
bovine serum albumin and dithiothreitol stabilize the enzyme during activity assay Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5'-AMP weak Homo sapiens
5'-Hydroxyl poly(I) in combination with 5'-hydroxyl poly(A) or poly(C) Homo sapiens
ADP
-
 Homo sapiens
Cu2+ inhibits in presence of MgCl2 Homo sapiens
diphosphate
-
 Homo sapiens
KCl weak inhibition, 50% at about 0.3 M Homo sapiens
Mn2+ inhibition above 1 mM Homo sapiens
N-ethylmaleimide
-
 Homo sapiens
NaCl weak inhibition, 50% at about 0.3 M Homo sapiens
NH4+ 50% at about 0.3 M; weak inhibition Homo sapiens
phosphate weak Homo sapiens
Ribonucleoside 3'-phosphates weak Homo sapiens
sulfate weak Homo sapiens
Zn2+ inhibition in presence of Mg2+ Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
 5'-hydroxyl poly(A) pH 8.4, 37°C Homo sapiens
0.0034
-
 5'-hydroxyl poly(C) pH 8.4, 37°C Homo sapiens
0.5
-
 ATP pH 8.4, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ divalent cation required, slight activation in absence of MgCl2 Homo sapiens
Ca2+ about 17% of the activity with Mn2+, synergistic to Mn2+ Homo sapiens
divalent cations absolutely required Homo sapiens
Mg2+ Mg2+ or Mn2+ effective in activation Homo sapiens
Mg2+ 86% of the activity with Mn2+ Homo sapiens
Mn2+ Mg2+ or Mn2+ effective in activation Homo sapiens
Mn2+ maximal activation at 0.6-1.0 mM, inhibition above 1 mM Homo sapiens
Zn2+ no activation, inhibition in presence of Mg2+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5'-dephospho-DNA Homo sapiens
-
 ADP + 5'-phospho-DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 HeLa cells
-

Purification (Commentary)

Purification (Comment) Organism
431fold Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
cell culture HeLa cells Homo sapiens
-
HeLa cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.125
-
 purified enzyme, substrate RNA Homo sapiens

Storage Stability

Storage Stability Organism
0°C, 10% loss of activity after 2 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5'-dephospho-DNA
-
 Homo sapiens ADP + 5'-phospho-DNA
-
 ?
ATP + 5'-dephospho-RNA much more efficient as substrate than DNA Homo sapiens ADP + 5'-phospho-RNA
-
 ?
ATP + 5'-hydroxyl poly(A)
-
 Homo sapiens ?
-
 ?
ATP + 5'-hydroxyl poly(C)
-
 Homo sapiens ?
-
 ?
ATP + synthetic oligonucleotide 5'-hydroxyl poly(A) Homo sapiens ADP + oligonucleotide 5'-phosphate
-
 ?
ATP + synthetic oligonucleotide 5'-hydroxyl poly(C) Homo sapiens ADP + oligonucleotide 5'-phosphate
-
 ?
ATP + synthetic oligonucleotide low activity with 5'-hydroxyl poly(I) Homo sapiens ADP + oligonucleotide 5'-phosphate
-
 ?
ATP + synthetic oligonucleotide 5'-hydroxyl poly(dA), at 6% of 5'-hydroxyl poly(A) Homo sapiens ADP + oligonucleotide 5'-phosphate
-
 ?
CTP + 5'-dephospho-DNA CTP gives 15% of the activity with ATP Homo sapiens CDP + 5'-phospho-DNA
-
 ?
CTP + 5'-dephospho-RNA less effective than ATP Homo sapiens CDP + 5'-phospho-RNA
-
 ?
GTP + 5'-dephospho-DNA GTP gives 15% of the activity with ATP Homo sapiens GDP + 5'-phospho-DNA
-
 ?
[gamma-S]GTP + 5'-dephospho-RNA less effective than ATP Homo sapiens [gamma-S]GDP + 5'-phospho-RNA
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 57% loss of RNA-dependent activity, 34% of DNA-dependent activity Homo sapiens
55
-
 5 min, complete loss of activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Homo sapiens
5.5
-
 substrate DNA Homo sapiens
7.9 8.9
-
 Homo sapiens
8.4
-
 assay at, substrate RNA Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.9 pH 6.5: about 50% of activity maximum, pH 7.9-8.9: activity maximum, substrate RNA Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Homo sapiens
ATP best at 2 mM Homo sapiens
CDP
-
 Homo sapiens
CTP
-
 Homo sapiens
GDP
-
 Homo sapiens
GTP
-
 Homo sapiens