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Literature summary for 2.7.1.78 extracted from
- A rapid purification of T4 polynucleotide kinase using Blue Dextran-Sepharose chromatography (1978), Biochim. Biophys. Acta, 526, 410-417.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cibacron blue F3GA | chromophore of blue dextran, inhibition is competitive to single stranded DNA, noncompetitive with respect to ATP | Tequatrovirus T4 |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0065 | - |
5'-OH-DNA | pH 7.4, 37°C | Tequatrovirus T4 | |
| 0.065 | - |
ATP | pH 7.4, 37°C | Tequatrovirus T4 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Tequatrovirus T4 | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5'-dephospho-DNA | Tequatrovirus T4 | - |
ADP + 5'-phospho-DNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tequatrovirus T4 | - |
from infected Escherichia coli | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to near homogeneity | Tequatrovirus T4 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.393 | - |
purified enzyme | Tequatrovirus T4 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5'-dephospho-DNA | - |
Tequatrovirus T4 | ADP + 5'-phospho-DNA | - |
? | |
| ATP + 5'-dephospho-DNA | single stranded DNA | Tequatrovirus T4 | ADP + 5'-phospho-DNA | - |
? | |
| ATP + 5'-dephospho-DNA | micrococcal-nuclease-treated calf thymus DNA | Tequatrovirus T4 | ADP + 5'-phospho-DNA | - |
? | |
| ATP + 5'-dephospho-RNA | - |
Tequatrovirus T4 | ADP + 5'-phospho-RNA | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Tequatrovirus T4 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Tequatrovirus T4 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on | Tequatrovirus T4 | |
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Release 2023.1 (January 2023)
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