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Literature summary for 2.7.1.78 extracted from
- Characterization of DNA kinase from calf thymus (1981), Eur. J. Biochem., 115, 449-453.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| polyamines | stimulate at suboptimal Mg2+ concentration | Bos taurus | |
| spermidine | 3-4fold activation, activates by lowering the Km for Mg2+ and enhancing Vmax, optimal at 10-15 mM | Bos taurus |  |
| spermine | activates 3-4fold at 2 mM | Bos taurus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ammonium sulfate | about 75% inhibition at 10 mM | Bos taurus | |
| diphosphate | weak | Bos taurus | |
| p-chloromercuribenzoate | reversible | Bos taurus | |
| sulfate | weak | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
ATP | pH 5.5, 37°C | Bos taurus | |
| 2.5 | - |
Mg2+ | pH 5.5, 37°C | Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | KCl slightly stimulates | Bos taurus | |
| Mg2+ | required | Bos taurus | |
| Mg2+ | optimal concentration is 10 mM | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
1 * 54000, SDS-PAGE | Bos taurus |
| 56000 | - |
gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5'-dephospho-DNA | Bos taurus | - |
ADP + 5'-phospho-DNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| thymus | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0167 | - |
purified enzyme | Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, stable for at least 1 month | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5'-dephospho-DNA | - |
Bos taurus | ADP + 5'-phospho-DNA | - |
? | |
| ATP + 5'-dephospho-RNA | no activity | Bos taurus | ADP + 5'-phospho-RNA | - |
? | |
| ATP + synthetic oligonucleotide | no activity with poly(A) | Bos taurus | ADP + oligonucleotide 5'-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 54000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 30 | - |
Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
sharp maximum | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on | Bos taurus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5 | - |
diphosphate | pH 5.5, 25°C | Bos taurus | |
| 7 | - |
sulfate | pH 5.5, 25°C | Bos taurus | |
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Release 2023.1 (January 2023)
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