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Literature summary for 2.7.1.78 extracted from

  • Lillehaug, J.R.
    Physicochemical properties of T4 polynucleotide kinase (1977), Eur. J. Biochem., 73, 499-506.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
spermine stabilizes the enzyme in an oligomeric state Tequatrovirus T4

Application

Application Comment Organism
biotechnology application in DNA and RNA sequencing Tequatrovirus T4
synthesis enzyme is an important tool in the synthesis of genes corresponding to yeast alanine tRNAand to precursor tyrosine tRNA of Escherichia coli Tequatrovirus T4

General Stability

General Stability Organism
high ionic strength, 0.1 M KCl, spermine, ATP and thymidine 3'-monophosphate stabilize the oligomeric structure Tequatrovirus T4
high ionic strength, e.g. 0.1 M KCl, spermine, ATP and thymidine 3'-monophosphate are essential to stabilize the enzyme in an oligomeric state Tequatrovirus T4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
4 * 33000, sedimentation analysis and amino acid sequence determination Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5'-dephospho-DNA
-
Tequatrovirus T4 ADP + 5'-phospho-DNA
-
?
ATP + deoxynucleoside 3'-phosphate
-
Tequatrovirus T4 ADP + deoxynucleoside 3',5'-diphosphate
-
?

Subunits

Subunits Comment Organism
More each monomer contains 2 SH-groups, one of which is more buried Tequatrovirus T4
More high ionic strength, e.g. 0.1 M KCl, spermine, ATP and thymidine 3'-monophosphate are essential to stabilize the enzyme in an oligomeric state Tequatrovirus T4
tetramer 4 * 33000, sedimentation analysis and amino acid sequence determination Tequatrovirus T4

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Tequatrovirus T4
ATP stabilizes the enzyme in an oligomeric state Tequatrovirus T4