Activating Compound | Comment | Organism | Structure |
---|---|---|---|
spermine | stabilizes the enzyme in an oligomeric state | Tequatrovirus T4 |
Application | Comment | Organism |
---|---|---|
biotechnology | application in DNA and RNA sequencing | Tequatrovirus T4 |
synthesis | enzyme is an important tool in the synthesis of genes corresponding to yeast alanine tRNAand to precursor tyrosine tRNA of Escherichia coli | Tequatrovirus T4 |
General Stability | Organism |
---|---|
high ionic strength, 0.1 M KCl, spermine, ATP and thymidine 3'-monophosphate stabilize the oligomeric structure | Tequatrovirus T4 |
high ionic strength, e.g. 0.1 M KCl, spermine, ATP and thymidine 3'-monophosphate are essential to stabilize the enzyme in an oligomeric state | Tequatrovirus T4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
4 * 33000, sedimentation analysis and amino acid sequence determination | Tequatrovirus T4 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | - |
Tequatrovirus T4 | ADP + 5'-phospho-DNA | - |
? | |
ATP + deoxynucleoside 3'-phosphate | - |
Tequatrovirus T4 | ADP + deoxynucleoside 3',5'-diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | each monomer contains 2 SH-groups, one of which is more buried | Tequatrovirus T4 |
More | high ionic strength, e.g. 0.1 M KCl, spermine, ATP and thymidine 3'-monophosphate are essential to stabilize the enzyme in an oligomeric state | Tequatrovirus T4 |
tetramer | 4 * 33000, sedimentation analysis and amino acid sequence determination | Tequatrovirus T4 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Tequatrovirus T4 | |
ATP | stabilizes the enzyme in an oligomeric state | Tequatrovirus T4 |