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Literature summary for 2.7.1.78 extracted from

  • Novogrodsky, A.; Hurwitz, J.
    The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid. I. Phosphorylation at 5-hydroxyl termini (1966), J. Biol. Chem., 241, 2923-2932.
    View publication on PubMed
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General Stability

General Stability Organism
freezing and thawing reduces activity after 1 cycle by 60% Tequatrovirus T4
freezing and thawing reduces activity after 1 cycle by 60% Escherichia phage T2

Inhibitors

Inhibitors Comment Organism Structure
chloramphenicol inhibits the formation of enzyme in cells cotransfected with bacteriophage and chloramphenicol Escherichia phage T2
chloramphenicol inhibits the formation of enzyme in cells cotransfected with bacteriophage and chloramphenicol Tequatrovirus T4

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Escherichia phage T2
Mg2+ required Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5'-dephospho-DNA Tequatrovirus T4
-
 ADP + 5'-phospho-DNA
-
 ?
ATP + 5'-dephospho-DNA Escherichia phage T2
-
 ADP + 5'-phospho-DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia phage T2
-
 from infected Escherichia coli
-
no activity in bacteriophage T1
-
-
-
no activity in bacteriophage T5
-
-
-
Tequatrovirus T4
-
 from infected Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia phage T2

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.53
-
 purified enzyme Tequatrovirus T4

Storage Stability

Storage Stability Organism
0°C, partially purified ammonium sulfate fraction, stable for more than 1 year Escherichia phage T2
0°C, purified protein, concentration 0.014 mg/ml, stable for 5 months Escherichia phage T2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'(3')-ribonucleotides no activity with 2'(3')-AMP and 2'(3')-CMP Escherichia phage T2 ?
-
 ?
ATP + 5'-dephospho-DNA
-
 Tequatrovirus T4 ADP + 5'-phospho-DNA
-
 ?
ATP + 5'-dephospho-DNA
-
 Escherichia phage T2 ADP + 5'-phospho-DNA
-
 ?
ATP + 5'-dephospho-DNA micrococcal-nuclease-treated calf thymus DNA Tequatrovirus T4 ADP + 5'-phospho-DNA
-
 ?
ATP + 5'-dephospho-DNA micrococcal-nuclease-treated calf thymus DNA Escherichia phage T2 ADP + 5'-phospho-DNA
-
 ?
ATP + 5'-dephospho-RNA
-
 Tequatrovirus T4 ADP + 5'-phospho-RNA
-
 ?
ATP + 5'-dephospho-RNA soluble and ribosomal RNA of Escherichia coli Escherichia phage T2 ADP + 5'-phospho-RNA
-
 ?
ATP + 5'-dephospho-RNA synthetic polynucleotides Escherichia phage T2 ADP + 5'-phospho-RNA
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
 assay at Tequatrovirus T4
38
-
 assay at Escherichia phage T2

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 marked temperature lability in absence of substrate Tequatrovirus T4
25
-
 marked temperature lability in absence of substrate Escherichia phage T2
37
-
-
 Escherichia phage T2
37
-
 15 min, 77% loss of activity Tequatrovirus T4
45
-
 5 min, loss of 94% activity with substrate DNA and 91% activity with substrate RNA Tequatrovirus T4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Tequatrovirus T4
6
-
 assay at Escherichia phage T2

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Tequatrovirus T4
ATP dependent on Escherichia phage T2