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Literature summary for 2.7.1.77 extracted from

  • Birichevskaya, L.L.; Kvach, S.V.; Sivets, G.G.; Kalinichenko, E.N.; Zinchenko, A.I.; Mikhailopulo, I.A.
    A comparison of enzymatic phosphorylation and phosphatidylation of beta-L- and beta-D-nucleosides (2007), Biotechnol. Lett., 29, 585-591.
    View publication on PubMed
Search for more literature for 2.7.1.77

Organism

Organism UniProt Comment Textmining
Pantoea agglomerans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
phenyl Sepharose CL-4B column chromatography Pantoea agglomerans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.23
-
 substrate beta-L-thymidine, pH 4.5, 37°C Pantoea agglomerans
0.4
-
 substrate beta-L-2',3'-dideoxycytidine, pH 4.5, 37°C Pantoea agglomerans
0.55
-
 substrate adenosine, pH 4.5, 37°C Pantoea agglomerans
0.57
-
 substrate beta-L-3'-deoxy-2',3'-didehydrothymidine, pH 4.5, 37°C Pantoea agglomerans
0.63
-
 substrate O2,2'-anhydro-beta-L-5-methyluridine, pH 4.5, 37°C Pantoea agglomerans
0.73
-
 substrate 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl) thymine, pH 4.5, 37°C Pantoea agglomerans
0.95
-
 substrate beta-L-3'-deoxy-3'-chlorothymidine, pH 4.5, 37°C Pantoea agglomerans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl) thymine 80% maximum conversion to 5'-monophosphate Pantoea agglomerans 4-nitrophenol + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl) thymine 5-phosphate
-
 ?
4-nitrophenyl phosphate + adenosine
-
 Pantoea agglomerans 4-nitrophenol + AMP
-
 ?
4-nitrophenyl phosphate + beta-L-2',3'-dideoxycytidine 38% maximum conversion to 5'-monophosphate Pantoea agglomerans 4-nitrophenol + beta-L-2',3'-dideoxycytidine 5'-phosphate
-
 ?
4-nitrophenyl phosphate + beta-L-3'-deoxy-2',3'-didehydrothymidine 60% maximum conversion to 5'-monophosphate Pantoea agglomerans 4-nitrophenol + beta-L-3'-deoxy-2',3'-didehydrothymidine 5'-phosphate
-
 ?
4-nitrophenyl phosphate + beta-L-3'-deoxy-3'-chlorothymidine 90% maximum conversion to 5'-monophosphate Pantoea agglomerans 4-nitrophenol + beta-L-3'-deoxy-3'-chlorothymidine 5'-phosphate
-
 ?
4-nitrophenyl phosphate + beta-L-thymidine 35% maximum conversion to 5'-monophosphate Pantoea agglomerans 4-nitrophenol + beta-L-thymidine 5'-phosphate
-
 ?
4-nitrophenyl phosphate + O2,2'-anhydro-beta-L-5-methyluridine 65% maximum conversion to 5'-monophosphate Pantoea agglomerans 4-nitrophenol + O2,2'-anhydro-beta-L-5-methyluridine 5'-phosphate
-
 ?
additional information no activity with L-adenosine, L-cytidine, L-ribofuranosylthymine, and 1-(beta-L-arabinofuranosyl)thymine Pantoea agglomerans ?
-
 ?
p-nitrophenylphosphate + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl)thymine
-
 Pantoea agglomerans 4-nitrophenol + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl)thymine 5'-phosphate
-
 ?
p-nitrophenylphosphate + beta-L-2',3'-dideoxycytidine
-
 Pantoea agglomerans 4-nitrophenol + beta-L-2',3'-dideoxycytidine 5'-phosphate
-
 ?
p-nitrophenylphosphate + L-3'-deoxy-2',3'-didehydrothymidine
-
 Pantoea agglomerans 4-nitrophenol + L-3'-deoxy-2',3'-didehydrothymidine 5'-phosphate
-
 ?
p-nitrophenylphosphate + L-3'-deoxy-3'-chlorothymidine
-
 Pantoea agglomerans 4-nitrophenol + L-3'-deoxy-3'-chlorothymidine 5'-phosphate
-
 ?
p-nitrophenylphosphate + L-thymidine
-
 Pantoea agglomerans 4-nitrophenol + L-thymidine 5'-phosphate
-
 ?
p-nitrophenylphosphate + O2,2'-anhydro-beta-L-5-methyluridine
-
 Pantoea agglomerans 4-nitrophenol + O2,2'-anhydro-beta-L-5-methyluridine 5'-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
NPT
-
 Pantoea agglomerans