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Literature summary for 2.7.1.76 extracted from

  • Sandrini, M.P.; Clausen, A.R.; On, S.L.; Aarestrup, F.M.; Munch-Petersen, B.; Piskur, J.
    Nucleoside analogues are activated by bacterial deoxyribonucleoside kinases in a species-specific manner (2007), J. Antimicrob. Chemother., 60, 510-520.
    View publication on PubMed
Search for more literature for 2.7.1.76

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Bacillus cereus
expression in Escherichia coli Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
 2'-deoxycytidine
-
 Bacillus cereus
0.027
-
 deoxycytidine in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS Bacillus cereus
0.0332
-
 2'-deoxyadenosine
-
 Bacillus cereus
0.0332
-
 deoxyadenosine in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS Bacillus cereus
0.0335
-
 gemcitabine in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS Bacillus cereus
0.0335
-
 2',2'-difluorodeoxycytidine ratio kcat/Km is 5100 per s and M Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26200
-
 SDS-PAGE Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus Q0H0H5
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose FF column chromatography Bacillus cereus
recombinant protein Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2',2'-difluorodeoxycytidine enzyme activates 2',2'-difluorodesoxycytidine in vivo Bacillus cereus ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
 ?
ATP + 2'-deoxyadenosine
-
 Bacillus cereus ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
ATP + 2'-deoxycytidine
-
 Bacillus cereus ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
ATP + deoxyadenosine
-
 Bacillus cereus ADP + dAMP
-
 ?
ATP + deoxycytidine
-
 Bacillus cereus ADP + dCMP
-
 ?
ATP + gemcitabine
-
 Bacillus cereus ADP + ?
-
 ?

Synonyms

Synonyms Comment Organism
dAK
-
 Bacillus cereus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.17
-
 gemcitabine in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS Bacillus cereus
0.55
-
 deoxycytidine in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS Bacillus cereus
1.22
-
 deoxyadenosine in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS Bacillus cereus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Bacillus cereus