BRENDA - Enzyme Database
show all sequences of 2.7.1.76

Selective inactivation of the deoxyadenosine phosphorylating activity of pure human deoxycytidine kinase: stabilization of different forms of the enzyme by substrates and biological detergents

Kierdaszuk, B.; Eriksson, S.; Biochemistry 29, 4109-4114 (1990)

Data extracted from this reference:

General Stability
General Stability
Organism
stability of deoxyadenosine kinase activity differs appreciably from deoxycytidine kinase activity
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
CHAPS
at critical micelle concentration
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic study
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + deoxyadenosine
Homo sapiens
key anabolic enzyme for activation of purine and pyrimidine deoxyribonucleosides as well as cytosine arabinoside and other anti-tumour drugs
ADP + 5'-dAMP
-
Homo sapiens
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
spleen
leukemic
Homo sapiens
-
Storage Stability
Storage Stability
Organism
4°C, t1/2: 84 h with and t1/2: 32 h without addition of bovine serum albumin
Homo sapiens
ATP, dTTP, deoxyadenosine or CHAPS stabilizes at 4°C
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + deoxyadenosine
-
641937
Homo sapiens
ADP + dAMP
-
641937
Homo sapiens
?
ATP + deoxyadenosine
key anabolic enzyme for activation of purine and pyrimidine deoxyribonucleosides as well as cytosine arabinoside and other anti-tumour drugs
641937
Homo sapiens
ADP + 5'-dAMP
-
641937
Homo sapiens
?
ATP + deoxycytidine
deoxycytidine kinase is a multisubstrate enzyme that also phosphorylates deoxyadenosine, it exists in different conformational states with different substrate kinetic properties
641937
Homo sapiens
ADP + dCMP
-
641937
Homo sapiens
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
44
-
-
Homo sapiens
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
27
50
approx. half-maximal activity at 27°C and 50°C
Homo sapiens
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
temperature stability of deoxyadenosine kinase activity differs appreciably from deoxycytidine kinase activity
Homo sapiens
4
-
half-life of deoxyadenosine kinase activity: 1.5 days
Homo sapiens
37
-
t1/2: 2 h, 1 mM ATP, 0.5 mM dTTP, 1 mM deoxyadenosine or 0.25 3-[(3-cholamidopropyl)dimethylamino]-1-propanesulfonate stabilize
Homo sapiens
48
-
inactivation of deoxyadenosine kinase activity
Homo sapiens
50
-
80% loss of activity
Homo sapiens
General Stability (protein specific)
General Stability
Organism
stability of deoxyadenosine kinase activity differs appreciably from deoxycytidine kinase activity
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CHAPS
at critical micelle concentration
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic study
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + deoxyadenosine
Homo sapiens
key anabolic enzyme for activation of purine and pyrimidine deoxyribonucleosides as well as cytosine arabinoside and other anti-tumour drugs
ADP + 5'-dAMP
-
Homo sapiens
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
spleen
leukemic
Homo sapiens
-
Storage Stability (protein specific)
Storage Stability
Organism
4°C, t1/2: 84 h with and t1/2: 32 h without addition of bovine serum albumin
Homo sapiens
ATP, dTTP, deoxyadenosine or CHAPS stabilizes at 4°C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + deoxyadenosine
-
641937
Homo sapiens
ADP + dAMP
-
641937
Homo sapiens
?
ATP + deoxyadenosine
key anabolic enzyme for activation of purine and pyrimidine deoxyribonucleosides as well as cytosine arabinoside and other anti-tumour drugs
641937
Homo sapiens
ADP + 5'-dAMP
-
641937
Homo sapiens
?
ATP + deoxycytidine
deoxycytidine kinase is a multisubstrate enzyme that also phosphorylates deoxyadenosine, it exists in different conformational states with different substrate kinetic properties
641937
Homo sapiens
ADP + dCMP
-
641937
Homo sapiens
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
44
-
-
Homo sapiens
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
27
50
approx. half-maximal activity at 27°C and 50°C
Homo sapiens
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
temperature stability of deoxyadenosine kinase activity differs appreciably from deoxycytidine kinase activity
Homo sapiens
4
-
half-life of deoxyadenosine kinase activity: 1.5 days
Homo sapiens
37
-
t1/2: 2 h, 1 mM ATP, 0.5 mM dTTP, 1 mM deoxyadenosine or 0.25 3-[(3-cholamidopropyl)dimethylamino]-1-propanesulfonate stabilize
Homo sapiens
48
-
inactivation of deoxyadenosine kinase activity
Homo sapiens
50
-
80% loss of activity
Homo sapiens
Other publictions for EC 2.7.1.76
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739204
Clausen
Plants salvage deoxyribonucleo ...
Arabidopsis thaliana, Solanum tuberosum
Nucleosides Nucleotides Nucleic Acids
33
291-295
2014
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2
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2
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6
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1
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2
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-
-
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-
-
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-
694483
Joachims
Inhibition of deoxynucleoside ...
Homo sapiens
Nucleosides Nucleotides Nucleic Acids
27
816-820
2008
-
1
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1
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1
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1
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674219
Sandrini
Nucleoside analogues are activ ...
Bacillus cereus
J. Antimicrob. Chemother.
60
510-520
2007
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1
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6
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1
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11
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1
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6
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3
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1
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6
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1
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6
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3
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675428
Welin
Structure-function analysis of ...
Mycoplasma mycoides
J. Mol. Biol.
366
1615-1623
2007
-
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1
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2
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2
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5
-
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1
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46
1
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1
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1
2
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2
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2
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1
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46
1
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-
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-
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-
-
675444
Sandrini
Dictyostelium discoideum salva ...
Dictyostelium discoideum
J. Mol. Biol.
369
653-664
2007
-
-
1
1
-
-
-
5
-
-
1
1
-
7
-
-
-
-
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-
-
-
10
1
-
-
-
6
-
-
-
1
-
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-
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-
1
1
1
-
-
-
-
-
6
-
-
1
1
-
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-
-
-
-
-
10
1
-
-
-
6
-
-
-
-
-
-
-
-
-
-
690459
Sandrini
Deoxyribonucleoside kinases ac ...
Staphylococcus aureus
Antimicrob. Agents Chemother.
51
2726-2732
2007
-
-
1
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-
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3
-
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1
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7
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3
1
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3
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1
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3
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1
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3
1
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3
-
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-
672159
Pol
Kinetic mechanism of deoxyaden ...
Mycoplasma mycoides
Biochemistry
45
513-522
2006
-
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10
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3
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6
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1
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1
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10
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6
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676722
Katahira
Profiles of purine biosynthesi ...
Solanum tuberosum
Planta
225
115-126
2006
-
-
-
-
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3
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1
1
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1
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1
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1
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1
1
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1
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-
660855
Krawiec
Inorganic tripolyphosphate (PP ...
Homo sapiens
Biochem. Biophys. Res. Commun.
301
192-197
2003
-
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1
4
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1
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2
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1
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2
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4
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1
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1
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2
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2
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1
2
4
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1
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2
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2
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4
-
1
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-
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1
-
-
-
-
-
-
-
-
-
662985
Krawiec
Ability of adenosine-2'(3')-de ...
Homo sapiens
Nucleosides Nucleotides Nucleic Acids
22
1525-1529
2003
-
-
-
-
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1
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2
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1
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2
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14
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7
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7
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1
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2
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2
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14
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640561
Andersen
Deoxynucleoside kinases encode ...
Bacillus subtilis
J. Biol. Chem.
276
5518-5524
2001
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1
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1
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641988
Wang
Novel deoxynucleoside-phosphor ...
Acholeplasma laidlawii, Mycoplasma arginini, Mycoplasma mycoides, Mycoplasma pneumoniae
Mol. Microbiol.
42
1065-1073
2001
-
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1
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7
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2
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12
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1
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10
1
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7
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1
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7
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2
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1
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10
1
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7
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641989
Park
-
Protein engineering of deoxynu ...
Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396
J. Food Sci. Nutr.
6
79-81
2001
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3
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641984
Jenuth
Modeling of purine nucleoside ...
Mus musculus
Biochem. Cell Biol.
74
219-225
1996
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1
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641987
Szabados
Metabolism of adenosine and de ...
Homo sapiens
Int. J. Biochem. Cell Biol.
28
1405-1414
1996
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2
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640565
Hong
Directed mutagenesis of deoxyg ...
Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396
J. Biol. Chem.
270
6602-6606
1995
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4
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5
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640567
Ma
Cloning and expression of the ...
Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396
J. Biol. Chem.
270
6595-6601
1995
1
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1
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1
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4
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1
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1
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4
1
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640566
Ikeda
Heterodimeric deoxynucleoside ...
Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396
Biochemistry
33
5328-5334
1994
-
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-
-
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1
4
-
-
-
2
-
-
2
-
-
1
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-
1
2
2
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
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2
-
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-
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1
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1
2
2
1
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641937
Kierdaszuk
Selective inactivation of the ...
Homo sapiens
Biochemistry
29
4109-4114
1990
-
-
-
-
-
1
1
1
-
-
-
1
-
1
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-
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-
-
1
-
2
3
-
1
1
5
-
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1
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1
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1
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1
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1
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2
3
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1
1
5
-
-
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-
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-
-
-
-
641980
Ikeda
Amino-terminal nucleotide-bind ...
Lactobacillus acidophilus
Biochemistry
27
8648-8652
1988
-
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3
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1
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1
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1
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10
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Streeter
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1974
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4
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2
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1
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1968
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11
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