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Literature summary for 2.7.1.76 extracted from
- Selective inactivation of the deoxyadenosine phosphorylating activity of pure human deoxycytidine kinase: stabilization of different forms of the enzyme by substrates and biological detergents (1990), Biochemistry, 29, 4109-4114.
General Stability
| General Stability | Organism |
|---|---|
| stability of deoxyadenosine kinase activity differs appreciably from deoxycytidine kinase activity | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CHAPS | at critical micelle concentration | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic study | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + deoxyadenosine | Homo sapiens | key anabolic enzyme for activation of purine and pyrimidine deoxyribonucleosides as well as cytosine arabinoside and other anti-tumour drugs | ADP + 5'-dAMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spleen | leukemic | Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, t1/2: 84 h with and t1/2: 32 h without addition of bovine serum albumin | Homo sapiens |
| ATP, dTTP, deoxyadenosine or CHAPS stabilizes at 4°C | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + deoxyadenosine | - |
Homo sapiens | ADP + dAMP | - |
? | |
| ATP + deoxyadenosine | key anabolic enzyme for activation of purine and pyrimidine deoxyribonucleosides as well as cytosine arabinoside and other anti-tumour drugs | Homo sapiens | ADP + 5'-dAMP | - |
? | |
| ATP + deoxycytidine | deoxycytidine kinase is a multisubstrate enzyme that also phosphorylates deoxyadenosine, it exists in different conformational states with different substrate kinetic properties | Homo sapiens | ADP + dCMP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 44 | - |
- |
Homo sapiens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | 50 | approx. half-maximal activity at 27°C and 50°C | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
temperature stability of deoxyadenosine kinase activity differs appreciably from deoxycytidine kinase activity | Homo sapiens |
| 4 | - |
half-life of deoxyadenosine kinase activity: 1.5 days | Homo sapiens |
| 37 | - |
t1/2: 2 h, 1 mM ATP, 0.5 mM dTTP, 1 mM deoxyadenosine or 0.25 3-[(3-cholamidopropyl)dimethylamino]-1-propanesulfonate stabilize | Homo sapiens |
| 48 | - |
inactivation of deoxyadenosine kinase activity | Homo sapiens |
| 50 | - |
80% loss of activity | Homo sapiens |
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