Literature summary for 2.7.1.74 extracted from

Muthu, P.; Chen, H.X.; Lutz, S.
Redesigning human 2'-deoxycytidine kinase enantioselectivity for L-nucleoside analogues as reporters in positron emission tomography (2014), ACS Chem. Biol., 9, 2326-2333.

Search for more literature for 2.7.1.74

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)pLysS cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with 5-methyldeoxycytidine and ADP, hanging drop vapor diffusion method	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
A100V/R104M/D133S	the mutant shows exquisitely high activity for D-deoxycytidine and D-thymidine	Homo sapiens
A100V/R104M/D133S/E196A	the mutant shows low activity for D-deoxycytidine and D-thymidine	Homo sapiens
A100V/R104M/D133S/E196L	inactive	Homo sapiens
A100V/R104M/D133S/F96D	inactive	Homo sapiens
A100V/R104M/D133S/F96Y	the mutant shows low activity for D-deoxycytidine and D-thymidine	Homo sapiens
A100V/R104M/D133S/W58E	inactive	Homo sapiens
A100V/R104M/D133S/W58V	the mutant shows low activity for D-deoxycytidine and D-thymidine	Homo sapiens
R104M/D133N	the mutant shows low activity for D-deoxycytidine and D-thymidine	Homo sapiens
R104M/D133N/A138I	inactive	Homo sapiens
R104M/D133N/L102Y	the mutant shows low activity for D-deoxycytidine and D-thymidine	Homo sapiens
R104M/D133N/L191A	the mutant shows low activity for D-deoxycytidine and D-thymidine	Homo sapiens
R104M/D133N/M85Y	the mutant shows low activity for D-deoxycytidine and D-thymidine	Homo sapiens
R104M/D133N/P89F	inactive	Homo sapiens
R104M/D133N/V130T	the mutant shows low activity for D-deoxycytidine and D-thymidine	Homo sapiens
R104M/D133N/V130T/L191A	the mutant shows low activity for D-deoxycytidine and D-thymidine	Homo sapiens
R104M/D133N/V55E	the mutant shows very low activity for D-deoxycytidine and D-thymidine	Homo sapiens
R104M/D133N/V55F	the mutant shows very low activity for D-deoxycytidine and D-thymidine	Homo sapiens
R104M/D133N/V55F/V130T	the mutant shows very low activity for D-deoxycytidine and D-thymidine	Homo sapiens
R104M/D133N/V55F/V130T/L191A	the mutant shows very low activity for D-deoxycytidine and D-thymidine	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P27707	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose resin column chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + deoxycytidine	-	Homo sapiens	ADP + dCMP	-	?
ATP + thymidine	-	Homo sapiens	ADP + L-dTMP	-	?

Synonyms

Synonyms	Comment	Organism
2'-deoxycytidine kinase	-	Homo sapiens
dCK	-	Homo sapiens

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Release 2023.1 (January 2023)