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Literature summary for 2.7.1.74 extracted from

  • Yang, C.; Lee, M.; Hao, J.; Cui, X.; Guo, X.; Smal, C.; Bontemps, F.; Ma, S.; Liu, X.; Engler, D.; Parker, W.B.; Xu, B.
    Deoxycytidine kinase regulates the G2/M checkpoint through interaction with cyclin-dependent kinase 1 in response to DNA damage (2012), Nucleic Acids Res., 40, 9621-9632.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.74

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is activated in response to DNA damage. The ataxia-telangiectasia-mutated (ATM) kinase phosphorylates dCK on Serine 74 to activate it in response to DNA damage Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information stable enzyme knockout in HeLa cells by expression of dCK-siRNA or shRNA Homo sapiens
S74A site-directed mutagenesis Homo sapiens
S74E site-directed mutagenesis Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens dCK interacts with cyclin-dependent kinase 1 (Cdk1) and the interaction inhibits Cdk1 activity both in vitro and in vivo ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the ataxia-telangiectasia-mutated kinase phosphorylates the enzyme on Ser74 to activate it in response to DNA damage, required in vivo and in vitro. Ser74 phosphorylation is required for initiation of the G2/M checkpoint Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
GM0536 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information dCK interacts with cyclin-dependent kinase 1 (Cdk1) and the interaction inhibits Cdk1 activity both in vitro and in vivo Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
dCK
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function deoxycytidine kinase (dCK) is a rate limiting enzyme critical for phosphorylation of endogenous deoxynucleosides for DNA synthesis and exogenous nucleoside analogues for anticancer and antiviral drug actions. The enzyme is activated in response to DNA damage. Deoxycytidine kinase regulates the G2/M checkpoint through interaction with cyclin-dependent kinase 1 in response to DNA damagereqquiring its phosphorylation at Ser74, overview Homo sapiens