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Literature summary for 2.7.1.74 extracted from

  • Hazra, S.; Szewczak, A.; Ort, S.; Konrad, M.; Lavie, A.
    Post-translational phosphorylation of serine 74 of human deoxycytidine kinase favors the enzyme adopting the open conformation making it competent for nucleoside binding and release (2011), Biochemistry, 50, 2870-2880.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.74

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain C41(DE3) Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme mutant C9S/C45S/C59S/S74E/R104M/D133A/C146S in complex with L-dT and UDP, hanging drop vapour diffusion method, mixing of 0.001 ml of 9-15 mg/ml protein mixed with 0.001 ml reservoir solution containing 0.9-1.5 M trisodium citrate dihydrate and 100 mM Tris, pH 7.5, room temperature, X-ray diffraction structure determination and analysis at 1.9-2.5 A resolution Homo sapiens

Protein Variants

Protein Variants Comment Organism
C9S/C45S/C59S/S74E/R104M/D133A/C146S site-directed mutagenesis, crystallization mutant Homo sapiens
D133A site-directed mutagenesis, the mutation renders the enzyme capable of thymidine binding Homo sapiens
R104M site-directed mutagenesis, the mutation renders the enzyme capable of thymidine binding Homo sapiens
S74E site-directed mutagenesis, replacing the serine with a glutamic acid mimic phosphorylation of Ser74, the enzyme containing the S74E mutation adopts the open state, but wild-type dCK can adopt the open state also in the absence of the S74E mutation Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic properties of the enzyme are modulated in vivo by phosphorylation of Ser74, mechanism, overview Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + deoxycytidine Homo sapiens
-
 ADP + dCMP
-
 ?
UTP + deoxycytidine Homo sapiens
-
 UDP + dCMP
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P27707
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein kinetic properties of the enzyme are modulated in vivo by phosphorylation of Ser74, conformational changes and mechanism, overview Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain C41(DE3) by nickel affinity chromatography and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deoxycytidine
-
 Homo sapiens ADP + dCMP
-
 ?
UTP + deoxycytidine
-
 Homo sapiens UDP + dCMP
-
 ?

Synonyms

Synonyms Comment Organism
dCK
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens
UTP
-
 Homo sapiens

General Information

General Information Comment Organism
additional information structure-function analysis, comparison of wild-type enzyme and mutant S74E Homo sapiens
physiological function phosphorylation of Ser74 might be a mechanism for regulating the specificity of the kinase to favor deoxycytidine Homo sapiens