Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.74 extracted from

  • Sabini, E.; Hazra, S.; Konrad, M.; Lavie, A.
    Elucidation of different binding modes of purine nucleosides to human deoxycytidine kinase (2008), J. Med. Chem., 51, 4219-4225.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.74

Crystallization (Commentary)

Crystallization (Comment) Organism
structural studies of ternary complexes. The enzyme conformation adjusts to the different hydrogen-bonding properties between 2'-deoxyadenosine and 2'-deoxyguanosine and to the presence of substituent at the 2-position present in 2'-deoxyguanosine and cladribine. The carbonyl group in 2'-deoxyguansoine elicits a conformational adjustment of the active site residues Arg104 and Asp133. 2'-Deoxyguanosine and cladribine adopt the anti conformation, in contrast to the syn conformation observed with 2'-deoxyadenosine Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0051
-
 2-chlorodeoxyadenosine cosubstrate UTP, pH 7.5, 37°C Homo sapiens
0.0085
-
 2'-deoxyadenosine cosubstrate UTP, pH 7.5, 37°C Homo sapiens
0.041
-
 2'-deoxyguanosine cosubstrate UTP, pH 7.5, 37°C Homo sapiens
0.078
-
 2-chlorodeoxyadenosine cosubstrate ATP, pH 7.5, 37°C Homo sapiens
0.115
-
 2'-deoxyadenosine cosubstrate ATP, pH 7.5, 37°C Homo sapiens
0.181
-
 2'-deoxyguanosine cosubstrate ATP, pH 7.5, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P27707
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxyadenosine
-
 Homo sapiens ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
ATP + 2'-deoxyguanosine
-
 Homo sapiens ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
ATP + 2-chlorodeoxyadenosine i.e. cladribine Homo sapiens ADP + 2-chlorodeoxyadenosine 5'-phosphate
-
 ?
UTP + 2'-deoxyadenosine
-
 Homo sapiens UDP + 2'-deoxyadenosine 5'-phosphate
-
 ?
UTP + 2'-deoxyguanosine
-
 Homo sapiens UDP + 2'-deoxyguanosine 5'-phosphate
-
 ?
UTP + 2-chlorodeoxyadenosine i.e. cladribine Homo sapiens UDP + 2-chlorodeoxyadenosine 5'-phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.37
-
 2'-deoxyadenosine cosubstrate UTP, pH 7.5, 37°C Homo sapiens
0.37
-
 2-chlorodeoxyadenosine cosubstrate UTP, pH 7.5, 37°C Homo sapiens
0.53
-
 2'-deoxyguanosine cosubstrate UTP, pH 7.5, 37°C Homo sapiens
0.6
-
 2-chlorodeoxyadenosine cosubstrate ATP, pH 7.5, 37°C Homo sapiens
2.1
-
 2'-deoxyadenosine cosubstrate ATP, pH 7.5, 37°C Homo sapiens
2.5
-
 2'-deoxyguanosine cosubstrate ATP, pH 7.5, 37°C Homo sapiens