Cloned (Comment) | Organism |
---|---|
expressed as His-tag fusion protein in HEK293T cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S11A | no significant effect on activity | Homo sapiens |
S11E | no significant effect on activity | Homo sapiens |
S15A | no significant effect on activity | Homo sapiens |
S15E | no significant effect on activity | Homo sapiens |
S74A | mutation markedly decreases activity | Homo sapiens |
S74E | mutation does not significantly modify dCK activity | Homo sapiens |
T3A | no significant effect on activity | Homo sapiens |
T3E | no significant effect on activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
lambda protein phosphatase | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00016 | - |
2'-deoxycytidine | with ATP as cosubstrate | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 34000, SDS-PAGE, native mass by gel filtration | Homo sapiens |
68000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxyadenosine | Homo sapiens | rate-limiting reaction in the salvage of deoxyribonucleosides | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 2'-deoxycytidine | Homo sapiens | rate-limiting reaction in the salvage of deoxyribonucleosides | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
ATP + 2'-deoxyguanosine | Homo sapiens | rate-limiting reaction in the salvage of deoxyribonucleosides | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
additional information | Homo sapiens | catalyzes the rate-limiting step of the deoxyribonucleoside salvage pathway, key role in the activation of numerous nucleoside analogues used in anti-cancer and antiviral chemotherapy | ? | - |
? | |
NTP + 1-beta-D-arabinosylcytosine | Homo sapiens | i.e. cytaribine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies | NDP + 1-beta-D-arabinosylcytosine 5'-phosphate | - |
? | |
NTP + 2',2'-difluorodeoxycytidine | Homo sapiens | i.e. gemcitabine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of solid malignant tumors | NDP + 2',2'-difluorodeoxycytidine 5'-phosphate | - |
? | |
NTP + 2',3'-dideoxycytidine | Homo sapiens | i.e. zalcitabine, activation of the pharmaceutically relevant substrate which is used in the treatment of HIV infections | NDP + 2',3'-dideoxycytidine 5'-phosphate | - |
? | |
NTP + 2'-deoxy-3'-thiacytidine | Homo sapiens | i.e. lamivudine, activation of the pharmaceutically relevant substrate which is used in the treatment of HIV infections | NDP + 2'-deoxy-3'-thiacytidine 5'-phosphate | - |
? | |
NTP + 2-chloro-2'-deoxyadenosine | Homo sapiens | i.e. cladribine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies | NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate | - |
? | |
NTP + 9-beta-D-arabinosyl 2-fluoroadenine | Homo sapiens | i.e. fludarabine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies | NDP + 9-beta-D-arabinosyl 2-fluoroadenine 5'-phosphate | - |
? | |
UTP + 2'-deoxyadenosine | Homo sapiens | rate-limiting reaction in the salvage of deoxyribonucleosides | UDP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
UTP + 2'-deoxycytidine | Homo sapiens | rate-limiting reaction in the salvage of deoxyribonucleosides | UDP + 2'-deoxycytidine 5'-phosphate | - |
? | |
UTP + 2'-deoxyguanosine | Homo sapiens | rate-limiting reaction in the salvage of deoxyribonucleosides | UDP + 2'-deoxyguanosine 5'-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27707 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | at least four residues phosphorylated, Thr-3, Ser-11, Ser-15, and Ser-74, Ser-74 phosphorylation level important for enzyme activity | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
CCRF-CEM cell | - |
Homo sapiens | - |
leukemia cell | - |
Homo sapiens | - |
lymphoid cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxyadenosine | rate-limiting reaction in the salvage of deoxyribonucleosides | Homo sapiens | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 2'-deoxycytidine | - |
Homo sapiens | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
ATP + 2'-deoxycytidine | rate-limiting reaction in the salvage of deoxyribonucleosides | Homo sapiens | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
ATP + 2'-deoxyguanosine | rate-limiting reaction in the salvage of deoxyribonucleosides | Homo sapiens | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
additional information | catalyzes the rate-limiting step of the deoxyribonucleoside salvage pathway, key role in the activation of numerous nucleoside analogues used in anti-cancer and antiviral chemotherapy | Homo sapiens | ? | - |
? | |
NTP + 1-beta-D-arabinosylcytosine | i.e. cytaribine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies | Homo sapiens | NDP + 1-beta-D-arabinosylcytosine 5'-phosphate | - |
? | |
NTP + 2',2'-difluorodeoxycytidine | i.e. gemcitabine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of solid malignant tumors | Homo sapiens | NDP + 2',2'-difluorodeoxycytidine 5'-phosphate | - |
? | |
NTP + 2',3'-dideoxycytidine | i.e. zalcitabine, activation of the pharmaceutically relevant substrate which is used in the treatment of HIV infections | Homo sapiens | NDP + 2',3'-dideoxycytidine 5'-phosphate | - |
? | |
NTP + 2'-deoxy-3'-thiacytidine | i.e. lamivudine, activation of the pharmaceutically relevant substrate which is used in the treatment of HIV infections | Homo sapiens | NDP + 2'-deoxy-3'-thiacytidine 5'-phosphate | - |
? | |
NTP + 2-chloro-2'-deoxyadenosine | i.e. cladribine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies | Homo sapiens | NDP + 2-chloro-2'-deoxyadenosine 5'-phosphate | - |
? | |
NTP + 9-beta-D-arabinosyl 2-fluoroadenine | i.e. fludarabine, activation of the pharmaceutically relevant substrate which is commonly used in the treatment of lymphoproliferative malignancies | Homo sapiens | NDP + 9-beta-D-arabinosyl 2-fluoroadenine 5'-phosphate | - |
? | |
UTP + 2'-deoxyadenosine | rate-limiting reaction in the salvage of deoxyribonucleosides | Homo sapiens | UDP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
UTP + 2'-deoxycytidine | rate-limiting reaction in the salvage of deoxyribonucleosides | Homo sapiens | UDP + 2'-deoxycytidine 5'-phosphate | - |
? | |
UTP + 2'-deoxyguanosine | rate-limiting reaction in the salvage of deoxyribonucleosides | Homo sapiens | UDP + 2'-deoxyguanosine 5'-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34000, SDS-PAGE, native mass by gel filtration | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
dCK | - |
Homo sapiens |