Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.74 extracted from

  • Al-Madhoun, A.S.; Eriksson, S.; Wang, Z.X.; Naimi, E.; Knaus, E.E.; Wiebe, L.I.
    Phosphorylation of isocarbostyril- and difluorophenyl-nucleoside thymidine mimics by the human deoxynucleoside kinases (2004), Nucleosides Nucleotides Nucleic Acids, 23, 1865-1874.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
2'-deoxycytidine pH 7.6, 37°C, recombinant enzyme Homo sapiens
0.0013
-
2,5-difluoro-4-[1-(2-deoxy-beta-L-ribofuranosyl)]-aniline pH 7.6, 37°C, recombinant enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2'-deoxycytidine Homo sapiens
-
ADP + 2'-deoxycytidine 5'-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
catalytic efficiencies of the purified recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-(2-deoxy-beta-D-ribofuranosyl)-isocarbostyril poor substrate, 1.4% of the activity with 2'-deoxycytidine Homo sapiens ADP + ?
-
?
ATP + 2'-deoxycytidine
-
Homo sapiens ADP + 2'-deoxycytidine 5'-phosphate
-
?
ATP + 2,5-difluoro-4-[1-(2-deoxy-beta-L-ribofuranosyl)]-aniline nucleoside mimic, 14.6% of the activity with 2'-deoxycytidine Homo sapiens ADP + ?
-
?
additional information no activity with several difluorophenyl nucleoside analogues and with 1-(2-deoxy-beta-D-ribofuranosyl)-7-iodoisocarbostyril, overview Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
dCK
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
2,5-difluoro-4-[1-(2-deoxy-beta-L-ribofuranosyl)]-aniline pH 7.6, 37°C, recombinant enzyme Homo sapiens
0.11
-
2'-deoxycytidine pH 7.6, 37°C, recombinant enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens