General Stability | Organism |
---|---|
dithiothreitol, MgCl2, ATP, KCl and glycerol stabilize | Homo sapiens |
very unstable at low protein concentrations | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-chloroadenosine | - |
Homo sapiens | |
Ba2+ | - |
Homo sapiens | |
Cu2+ | - |
Homo sapiens | |
cytidine | deoxycytidine as substrate | Homo sapiens | |
cytosine arabinoside | deoxycytidine as substrate | Homo sapiens | |
deoxyadenosine | deoxycytidine | Homo sapiens | |
deoxyguanosine | deoxycytidine as substrate | Homo sapiens | |
Dideoxycytidine | - |
Homo sapiens | |
HgCl2 | - |
Homo sapiens | |
additional information | little or no inhibition by AMP, dTMP, adenosine, guanosine; little or no inhibition by deoxyuridine; little or no inhibition by ribavirin; no inhibition by inosine, xanthosine, S-adenosylhomocysteine, S-adenosylmethionine; no inhibition by uridine, thymidine; overview | Homo sapiens | |
Nucleosides | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | 0.0015 | deoxycytidine | 37°C, pH 7.0 | Homo sapiens | |
0.02 | 0.04 | cytosine arabinoside | pH 7.0, 37°C | Homo sapiens | |
0.33 | 0.43 | deoxyguanosine | 37°C, pH 7.0 | Homo sapiens | |
0.45 | - |
cytidine | 37°C, pH 7.0 | Homo sapiens | |
0.5 | - |
deoxyadenosine | 37°C, pH 7.0 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation | Homo sapiens | |
Co2+ | activation | Homo sapiens | |
Co2+ | about 30% as efficient as Mg2+ | Homo sapiens | |
Fe2+ | activation | Homo sapiens | |
Fe2+ | about 60% as efficient as Mg2+ | Homo sapiens | |
Mg2+ | - |
Homo sapiens | |
Mn2+ | requirement | Homo sapiens | |
Mn2+ | about 90% as efficient as Mg2+ | Homo sapiens | |
additional information | no activation by Ba2+, Cu2+ | Homo sapiens | |
additional information | no activation by monovalent cations | Homo sapiens | |
Ni2+ | not | Homo sapiens | |
Zn2+ | activation | Homo sapiens | |
Zn2+ | about 30% as efficient as Mg2+ | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30500 | - |
2 * 30500, SDS-PAGE | Homo sapiens |
60000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leukemia cell | leukemic T-lymphoblasts | Homo sapiens | - |
leukemia cell | cell line MOLT-4 | Homo sapiens | - |
MOLT-4 cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4 | - |
cytidine as substrate, 37°C, pH 7.0 | Homo sapiens |
7.2 | - |
deoxyguanosine as substrate, 37°C, pH 7.0 | Homo sapiens |
8 | - |
37°C, pH 7.0 | Homo sapiens |
13.5 | - |
deoxyadenosine as substrate, 37°C, pH 7.0 | Homo sapiens |
Storage Stability | Organism |
---|---|
-85°C, in 1 mg/ml bovine serum albumin, 20% v/v glycerol, 0.2 M potassium phosphate, 2 mM ATP, 2.4 mM MgCl2 and 0.025 M DTT, more than 12 months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxycytidine | - |
Homo sapiens | ADP + 5'-dCMP | - |
? | |
ATP + cytidine | - |
Homo sapiens | ADP + ? | - |
? | |
CTP + 2'-deoxycytidine | poor | Homo sapiens | CDP + 2'-deoxy-CMP | - |
? | |
dGTP + 2'-deoxycytidine | best phosphate donor | Homo sapiens | dGDP + 2'-deoxy-CMP | - |
? | |
GTP + 2'-deoxycytidine | as good as ATP | Homo sapiens | GDP + 2'-deoxy-CMP | - |
? | |
NTP + cytosine arabinoside | - |
Homo sapiens | NDP + cytosine arabinoside 5'-monophosphate | - |
? | |
NTP + deoxyadenosine | - |
Homo sapiens | NDP + dAMP | - |
? | |
NTP + deoxycytidine | enzyme shows little specificity towards phosphate donor | Homo sapiens | NDP + dCMP | - |
? | |
NTP + deoxycytidine | no substrate: nucleoside diphosphates and their deoxy derivatives | Homo sapiens | NDP + dCMP | - |
? | |
NTP + deoxyguanosine | - |
Homo sapiens | NDP + dGMP | - |
? | |
UTP + 2'-deoxycytidine | poor | Homo sapiens | UDP + 2'-deoxy-CMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30500, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
broad | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 10 | about half-maximal activity at pH 5.8 and pH 10, about 90% of maximal activity at pH 6.5 and pH 9 | Homo sapiens |