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Literature summary for 2.7.1.74 extracted from

  • Wang, L.M.; Kucera, G.L.; Capizzi, R.L.
    Purification and characterization of deoxycytidine kinase from acute myeloid leukemia cell mitochondria (1993), Biochim. Biophys. Acta, 1202, 309-316.
    View publication on PubMed
Search for more literature for 2.7.1.74

Activating Compound

Activating Compound Comment Organism Structure
deoxyadenosine activation, deoxycytidine as substrate, mitochondrial isozyme Homo sapiens
deoxyguanosine activation, deoxycytidine as substrate, mitochondrial isozyme Homo sapiens
TTP stimulation, cytosolic isozyme I Homo sapiens
UDP stimulation, mitochondrial isozyme Homo sapiens
Uridine arabinoside activation, deoxycytidine as substrate, mitochondrial isozyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Cytosine arabinoside triphosphate strong Homo sapiens
dCMP only cytosolic isozyme I; product inhibition, strong Homo sapiens
dCTP cytosine arabinoside as substrate; isozyme II Homo sapiens
deoxyadenosine cytosolic isozyme I, weak Homo sapiens
deoxycytidine cytosine arabinoside as substrate Homo sapiens
deoxyguanosine deoxyguanosine as substrate, cytosolic isozyme I, weak Homo sapiens
deoxythymidine weak Homo sapiens
dGDP not Homo sapiens
dTTP only mitochondrial isozyme; strong Homo sapiens
dUDP only cytosolic isozyme I; strong Homo sapiens
GTP not, deoxyadenosine as substrate Homo sapiens
UDP only cytosolic isozyme I; strong Homo sapiens
Uridine arabinoside weak, cytosolic isozyme I Homo sapiens
UTP not mitochondrial isozyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 cytosine arabinoside deoxycytidine, cytosolic isozyme I, pH 7.5, 37°C Homo sapiens
0.007
-
 cytosine arabinoside cytosolic isozyme I, pH 7.5, 37°C Homo sapiens
0.014 0.0167 deoxycytidine pH 7.5, 37°C Homo sapiens
0.014 0.0167 deoxycytidine mitochondrial isozyme, pH 7.5, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol 2 isozymes: I and II Homo sapiens 5829
-
mitochondrion outer membrane Homo sapiens 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 1 * 52000, SDS-PAGE, mitochondrial isozyme Homo sapiens
70000
-
 human cytoplasmic isozymes Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Homo sapiens
mitochondrial isozyme (extracted with digitonin) Homo sapiens
partial Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
BG-1 cell
-
 Homo sapiens
-
leukemia cell from peripheral blood Homo sapiens
-
leukemia cell from acute myeloid leukemia (AML) patients Homo sapiens
-
leukemia cell ML-1 cells Homo sapiens
-
ML-1 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.048
-
 mitochondrial isozyme, pH 7.5, 37°C Homo sapiens
0.072
-
 cytoplasmic isozyme I, pH 7.5, 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxycytidine best phosphate donor cytosolic isozyme II and mitochondrial isozyme Homo sapiens ADP + 5'-dCMP
-
 ?
CTP + 2'-deoxycytidine as good as GTP, UTP, dATP, mitochondrial isozyme Homo sapiens CDP + 2'-deoxy-CMP
-
 ?
dGTP + 2'-deoxycytidine cytosolic isozyme I Homo sapiens dGDP + 2'-deoxy-CMP
-
 ?
dGTP + 2'-deoxycytidine poor substrate of mitochondrial isozyme Homo sapiens dGDP + 2'-deoxy-CMP
-
 ?
dUTP + 2'-deoxycytidine best phosphate donor, cytosolic enzyme I, poor, mitochondrial isozyme Homo sapiens dUDP + 2'-deoxy-CMP
-
 ?
GTP + 2'-deoxycytidine as good as dGTP, dTTP or dUTP, cytosolic isozyme I Homo sapiens GDP + 2'-deoxy-CMP
-
 ?
GTP + 2'-deoxycytidine as good as CTP, dATP or UTP, mitochondrial isozyme Homo sapiens GDP + 2'-deoxy-CMP
-
 ?
additional information several isozymes: cytosolic deoxycytidine kinase I and II, plus mitochondrial isozyme Homo sapiens ?
-
 ?
NTP + 2',2'-difluoro-2'-deoxycytidine cytosolic isozyme I, not mitochondrial isozyme Homo sapiens NDP + 2',2'-difluoro-2'-deoxycytidine 5'-phosphate
-
 ?
NTP + cytosine arabinoside D-isomer Homo sapiens NDP + cytosine arabinoside 5'-monophosphate
-
 ?
NTP + cytosine arabinoside cytosolic isozyme I Homo sapiens NDP + cytosine arabinoside 5'-monophosphate
-
 ?
NTP + cytosine arabinoside no acceptor substrate for cytosolic isozyme II or mitochondrial isozyme Homo sapiens NDP + cytosine arabinoside 5'-monophosphate
-
 ?
NTP + deoxyadenosine cytosolic isozyme I Homo sapiens NDP + dAMP
-
 ?
NTP + deoxyadenosine not mitochondrial isozyme Homo sapiens NDP + dAMP
-
 ?
NTP + deoxycytidine no substrate: guanosine Homo sapiens NDP + dCMP
-
 ?
NTP + deoxycytidine poor substrate: arabinosyl-CTP Homo sapiens NDP + dCMP
-
 ?
NTP + deoxycytidine no substrate: uridine arabinoside, cytosine Homo sapiens NDP + dCMP
-
 ?
NTP + deoxycytidine no substrate: adenosine, uridine Homo sapiens NDP + dCMP
-
 ?
NTP + deoxyguanosine cytosolic isozyme I Homo sapiens NDP + dGMP
-
 ?
NTP + deoxyguanosine not mitochondrial isozyme Homo sapiens NDP + dGMP
-
 ?
NTP + deoxythymidine mitochondrial isozyme, better than deoxycytidine, not cytosolic isozyme I Homo sapiens NDP + dTMP
-
 ?
NTP + deoxyuridine mitochondrial isozyme, not cytosolic isozyme I Homo sapiens NDP + dUMP
-
 ?
UTP + 2'-deoxycytidine as good as CTP, GTP or dATP Homo sapiens UDP + 2'-deoxy-CMP
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 52000, SDS-PAGE, mitochondrial isozyme Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens