Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.74 extracted from

  • Krenitsky, T.A.; Tuttle, J.V.; Koszalka, G.W.; Chen, I.S.; Beacham, L.M.; Rideout, J.L.; Elion, G.B.
    Deoxycytidine kinase from calf thymus. Substrate and inhibitor specificity (1976), J. Biol. Chem., 251, 4055-4061.
    View publication on PubMed
Search for more literature for 2.7.1.74

Inhibitors

Inhibitors Comment Organism Structure
alpha-D-2'-Deoxythioguanosine
-
 Bos taurus
cytidine alpha-isomer Bos taurus
additional information no inhibition by ribavirin Bos taurus
N6-Phenyladenosine
-
 Bos taurus
Non-substrate inhibitors
-
 Bos taurus
Substrate derivatives overview Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-values of a variety of substrate derivatives Bos taurus
0.002
-
 cytosine arabinoside L-isomer, 25°C, pH 7.6 Bos taurus
0.02 0.04 cytosine arabinoside pH 7.6, 25°C Bos taurus
0.02 0.04 cytosine arabinoside D-isomer Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxycytidine
-
 Bos taurus ADP + 5'-dCMP
-
 ?
ATP + cytidine poor: beta-isomer Bos taurus ADP + ?
-
 ?
ATP + cytidine not: alpha-isomer Bos taurus ADP + ?
-
 ?
NTP + beta-D-2'-deoxythioguanosine not alpha-isomer Bos taurus NDP + ?
-
 ?
NTP + cytosine arabinoside i.e. 1-beta-arabinofuranosyl cytosine, L-isomer better substrate than D-isomer Bos taurus NDP + cytosine arabinoside 5'-monophosphate
-
 ?
NTP + deoxycytidine specificity Bos taurus NDP + dCMP
-
 ?
NTP + deoxycytidine no substrate: ribavirin Bos taurus NDP + dCMP
-
 ?
NTP + deoxycytidine beta-D-2'-deoxyribonucleosides are more efficient than corresponding beta-D-arabinonucleosides or beta-ribonucleosides, rather nonspecific for base moiety of nucleoside substrate Bos taurus NDP + dCMP
-
 ?
NTP + deoxycytidine no substrate: adenosine, uridine Bos taurus NDP + dCMP
-
 ?
NTP + deoxycytidine poor substrate: beta-5-azacytidine Bos taurus NDP + dCMP
-
 ?
NTP + deoxyguanosine
-
 Bos taurus NDP + dGMP
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Bos taurus