Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3)pLysS cells | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
streptomycin | at pH 7.5 and 25°C | Escherichia coli | |
1.03 | - |
ATP | at pH 7.5 and 25°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
1 * 30000, SDS-PAGE | Escherichia coli |
31400 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + streptomycin | Escherichia coli | - |
ADP + streptomycin 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + streptomycin | - |
Escherichia coli | ADP + streptomycin 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
aminoglycoside phosphotransferase | - |
Escherichia coli |
APH(6)-Id | - |
Escherichia coli |
streptomycin phosphotransferase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
ATP | at pH 7.5 and 25°C | Escherichia coli | |
1.7 | - |
streptomycin | at pH 7.5 and 25°C | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme confers resistance to streptomycin | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
streptomycin | at pH 7.5 and 25°C | Escherichia coli |