Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3)pLysS cells | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000153 | - |
shikimate | at pH 7.6 and 25°C | Staphylococcus aureus | |
0.224 | - |
ATP | at pH 7.6 and 25°C | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 25 mM used in assay conditions | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + shikimate | Staphylococcus aureus | - |
ADP + 3-phosphoshikimate | - |
? | |
ATP + shikimate | Staphylococcus aureus MRSA252 | - |
ADP + 3-phosphoshikimate | - |
? | |
ATP + shikimate | Staphylococcus aureus ATCC BAA1720 | - |
ADP + 3-phosphoshikimate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | Q6GGG1 | - |
- |
Staphylococcus aureus ATCC BAA1720 | Q6GGG1 | - |
- |
Staphylococcus aureus MRSA252 | Q6GGG1 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + shikimate | - |
Staphylococcus aureus | ADP + 3-phosphoshikimate | - |
? | |
ATP + shikimate | - |
Staphylococcus aureus MRSA252 | ADP + 3-phosphoshikimate | - |
? | |
ATP + shikimate | - |
Staphylococcus aureus ATCC BAA1720 | ADP + 3-phosphoshikimate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 20000, SDS-PAGE | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
AroK | - |
Staphylococcus aureus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 26 | after 8 h of incubation, the enzyme maintains its maximal activity in a range from 4 to 20°C, while at 25 and 26°C, it drops to 70% and 60%, respectively. A further increase of two degrees leads to the loss of 70% of the maximal activity, and at 30°C the enzyme is practically inactive | Staphylococcus aureus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Staphylococcus aureus | calculated from amino acid sequence | - |
5.1 |