Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Colwellia psychrerythraea |
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
General Stability | Organism |
---|---|
the urea concentration required for half-maximal denaturation is 1.5 mM | Colwellia psychrerythraea |
the urea concentration required for half-maximal denaturation is 2.5 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
shikimate | at pH 7.5 and 25°C | Colwellia psychrerythraea | |
0.153 | - |
ATP | at pH 7.5 and 25°C | Colwellia psychrerythraea | |
0.362 | - |
shikimate | at pH 7.5 and 25°C | Escherichia coli | |
0.5 | - |
ATP | at pH 7.5 and 25°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 15 mM used in assay conditions | Colwellia psychrerythraea | |
Mg2+ | 15 mM used in assay conditions | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + shikimate | Colwellia psychrerythraea | - |
ADP + 3-phosphoshikimate | - |
r | |
ATP + shikimate | Escherichia coli | - |
ADP + 3-phosphoshikimate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Colwellia psychrerythraea | - |
- |
- |
Escherichia coli | P0A6D7 | - |
- |
Purification (Comment) | Organism |
---|---|
His-Bind agarose resin column chromatography | Colwellia psychrerythraea |
His-Bind agarose resin column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + shikimate | - |
Colwellia psychrerythraea | ADP + 3-phosphoshikimate | - |
r | |
ATP + shikimate | - |
Escherichia coli | ADP + 3-phosphoshikimate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * about 20000, SDS-PAGE | Colwellia psychrerythraea |
? | x * about 20000, SDS-PAGE | Escherichia coli |
? | x * 19490, calculated from amino acid sequence | Colwellia psychrerythraea |
Synonyms | Comment | Organism |
---|---|---|
AroK | - |
Colwellia psychrerythraea |
AroK | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Colwellia psychrerythraea |
40 | - |
- |
Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 40 | the enzyme retains about 40% of its maximal activity at 10°C and 40°C | Colwellia psychrerythraea |
15 | 40 | the enzyme retains about 40% of its maximal activity at 15°C and 40°C | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
33 | - |
melting temperature | Colwellia psychrerythraea |
45 | - |
melting temperature | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
28.8 | - |
shikimate | at pH 7.5 and 25°C | Colwellia psychrerythraea | |
32.8 | - |
ATP | at pH 7.5 and 25°C | Colwellia psychrerythraea | |
55.86 | - |
shikimate | at pH 7.5 and 25°C | Escherichia coli | |
122.51 | - |
ATP | at pH 7.5 and 25°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
154 | - |
shikimate | at pH 7.5 and 25°C | Escherichia coli | |
214 | - |
ATP | at pH 7.5 and 25°C | Colwellia psychrerythraea | |
245 | - |
ATP | at pH 7.5 and 25°C | Escherichia coli | |
288 | - |
shikimate | at pH 7.5 and 25°C | Colwellia psychrerythraea |