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Literature summary for 2.7.1.71 extracted from

  • Bowen, J.R.; Kosuge, T.
    In vivo activity, purification, and characterization of shikimate kinase from Sorghum (1979), Plant Physiol., 64, 382-386.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.71

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 5 mM, 29% inhibition Sorghum bicolor
ADP 4 mM, 44% inhibition Sorghum bicolor
AMP 4 mM, 27% inhibition Sorghum bicolor
caffeic acid 1 mM, 44% inhibition Sorghum bicolor
Na2SO4 5 mM, 27% inhibition Sorghum bicolor
NaCl 250 mM, 50% inhibition Sorghum bicolor
p-coumaric acid 1 mM, 14% inhibition Sorghum bicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 ATP pH 9.0 Sorghum bicolor
0.2
-
 shikimate pH 9.0 Sorghum bicolor

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ divalent cation required, Mg2+ and Mn2+ are most effective, Ca2+ activates to a lesser extent Sorghum bicolor
Co2+ divalent cation required, Mg2+ and Mn2+ are most effective, Co2+ activates to a lesser extent Sorghum bicolor
Fe2+ divalent cation required, Mg2+ and Mn2+ are most effective, Fe2+ activates to a lesser extent Sorghum bicolor
Mg2+ divalent cation required, Mg2+ and Mn2+ are most effective. Optimal Mg2+ concentration is 10 mM Sorghum bicolor
Mn2+ divalent cation required, Mg2+ and Mn2+ are most effective Sorghum bicolor
Ni2+ divalent cation required, Mg2+ and Mn2+ are most effective, Ca2+ activates to a lesser extent Sorghum bicolor

Organism

Organism UniProt Comment Textmining
Sorghum bicolor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sorghum bicolor

Source Tissue

Source Tissue Comment Organism Textmining
shoot
-
 Sorghum bicolor
-
stem
-
 Sorghum bicolor
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.05718
-
-
 Sorghum bicolor

Storage Stability

Storage Stability Organism
-18°C, 30% v/v glycerol, stable for several months with gradual loss of activity Sorghum bicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + shikimate
-
 Sorghum bicolor ADP + shikimate 3-phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 or higher, in Tris-HCl buffer or bis-Tris propane buffer Sorghum bicolor

pH Range

pH Minimum pH Maximum Comment Organism
6.2 9 pH 6.2: about 20% of maximal activity in Tris propane buffer, pH 7.8: about 30% of maximal activity, pH 9.0: optimum Sorghum bicolor