KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
D-fructose | pH 6.9, 25°C | Leuconostoc mesenteroides | |
0.4 | - |
D-mannose | pH 6.9, 25°C | Leuconostoc mesenteroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
sucrose density gradient centrifugation | Leuconostoc mesenteroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | Leuconostoc mesenteroides | enzyme initiates the metabolism of D-fructose and D-mannose | ADP + D-fructose 6-phosphate | - |
? | |
ATP + D-mannose | Leuconostoc mesenteroides | enzyme initiates the metabolism of D-fructose and D-mannose | ADP + D-mannose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
a single enzyme catalyzes phosphorylation of D-fructose and D-mannose | - |
Purification (Comment) | Organism |
---|---|
- |
Leuconostoc mesenteroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
76 | - |
- |
Leuconostoc mesenteroides |
Storage Stability | Organism |
---|---|
0°C, 0.2 M (NH4)2SO4, pH 7.0, stable for several months | Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | D-mannose and D-fructose phosphorylated equally well | Leuconostoc mesenteroides | ADP + D-fructose 6-phosphate | - |
? | |
ATP + D-fructose | enzyme initiates the metabolism of D-fructose and D-mannose | Leuconostoc mesenteroides | ADP + D-fructose 6-phosphate | - |
? | |
ATP + D-glucose | no activity with D-glucose or L-glucose | Leuconostoc mesenteroides | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-mannose | enzyme initiates the metabolism of D-fructose and D-mannose | Leuconostoc mesenteroides | ADP + D-mannose 6-phosphate | - |
? | |
ATP + D-mannose | D-mannose and D-fructose phosphorylated equally well | Leuconostoc mesenteroides | ADP + mannose 6-phosphate | D-mannose 6-phosphate | ? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
reaction with D-mannose | Leuconostoc mesenteroides |
7 | 8.5 | reaction with D-fructose | Leuconostoc mesenteroides |