Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL-21(DE3) cells | Entamoeba histolytica |
Protein Variants | Comment | Organism |
---|---|---|
D214A | the mutant displays a complete loss of activity | Entamoeba histolytica |
D276A | the mutant displays a complete loss of activity | Entamoeba histolytica |
K147A | the mutant displays a complete loss of activity | Entamoeba histolytica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | faint staining in the cytosol | Entamoeba histolytica | 5829 | - |
plasma membrane | substantial staining at the plasma membrane | Entamoeba histolytica | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the activity of the enzyme is highest in the presence of Mn2+ and Mg2+ | Entamoeba histolytica | |
Mn2+ | the activity of the enzyme is highest in the presence of Mn2+ and Mg2+ | Entamoeba histolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | Entamoeba histolytica | - |
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | Entamoeba histolytica HM1:IMSS | - |
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | C4LSF7 | - |
- |
Entamoeba histolytica HM1:IMSS | C4LSF7 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography or glutathione-Sepharose column chromatography | Entamoeba histolytica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
trophozoite | - |
Entamoeba histolytica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate | weak activity | Entamoeba histolytica | ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate | weak activity | Entamoeba histolytica HM1:IMSS | ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
Entamoeba histolytica | ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
Entamoeba histolytica HM1:IMSS | ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
Entamoeba histolytica | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | preferred substrate | Entamoeba histolytica | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
Entamoeba histolytica HM1:IMSS | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | preferred substrate | Entamoeba histolytica HM1:IMSS | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate | - |
Entamoeba histolytica | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate | - |
Entamoeba histolytica HM1:IMSS | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 100000, GST-tagged enzyme, calculated from amino acid sequence | Entamoeba histolytica |
? | x * 120000, GST-tagged enzyme, SDS-PAGE | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
phosphatidylinositol 4-phosphate 5-kinase | - |
Entamoeba histolytica |
PIP5Kalpha | isoform | Entamoeba histolytica |
PIP5Kbeta | isoform | Entamoeba histolytica |
PIP5Kgamma | isoform | Entamoeba histolytica |
PIPKI | - |
Entamoeba histolytica |
PtdIns(4)P kinase | - |
Entamoeba histolytica |