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Literature summary for 2.7.1.68 extracted from

  • Kwok, F.; Humpage, A.; Cheng, C.H.K.
    Phosphatidylinositol phosphate 5-kinase: purification and inhibition studies (1996), Prep. Biochem. Biotechnol., 26, 1-19.
    View publication on PubMed
Search for more literature for 2.7.1.68

Inhibitors

Inhibitors Comment Organism Structure
2'(3')-O-(2,4,6-Trinitrophenyl)ATP competitive Ovis aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 ATP pH 7.4, 30°C Ovis aries

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Ovis aries 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, SDS-PAGE Ovis aries
130000
-
 gel filtration Ovis aries

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ovis aries

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.75
-
-
 Ovis aries

Storage Stability

Storage Stability Organism
4°C, about 10% loss of activity after overnight storage Ovis aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Ovis aries ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
GTP + 1-phosphatidyl-1D-myo-inositol 4-phosphate no activity Ovis aries GDP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer x * 60000, SDS-PAGE Ovis aries

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.055
-
 2'(3')-O-(2,4,6-Trinitrophenyl)ATP pH 7.4, 30°C Ovis aries