Literature summary for 2.7.1.67 extracted from

Chalupska, D.; Eisenreichova, A.; Rozycki, B.; Rezabkova, L.; Humpolickova, J.; Klima, M.; Boura, E.
Structural analysis of phosphatidylinositol 4-kinase IIIbeta (PI4KB) - 14-3-3 protein complex reveals internal flexibility and explains 14-3-3 mediated protection from degradation in vitro (2017), J. Struct. Biol., 200, 36-44 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 Star cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
isoform PI4KB in complex with 14-3-3 protein, sitting drop vapor diffusion method, using 0.1M MES pH6, 10% (w/v) PEG 8000, 20% (v/v) ethylene glycol	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi apparatus	-	Homo sapiens	5794	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
122000	-	analytical ultracentrifugation	Homo sapiens
130000	-	calculated from amino acid sequence	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 1-phosphatidyl-1D-myo-inositol	Homo sapiens	-	ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9UBF8	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	14-3-3 proteins bind isoform PI4KB upon its phosphorylation by protein kinase D. 14-3-3 proteins protect isoform PI4KB from proteolytic degradation in vitro	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography, Mono Q column chromatography and Superdex S200 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 1-phosphatidyl-1D-myo-inositol	-	Homo sapiens	ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 65000, calculated from amino acid sequence	Homo sapiens
homodimer	2 * 66000, analytical ultracentrifugation	Homo sapiens

Synonyms

Synonyms	Comment	Organism
phosphatidylinositol 4-kinase IIIbeta	-	Homo sapiens
PI4KB	-	Homo sapiens

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Release 2023.1 (January 2023)