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Literature summary for 2.7.1.67 extracted from
- Heterologous expression, purification, and functional analysis of the Plasmodium falciparum phosphatidylinositol 4-kinase IIIbeta (2020), Biochemistry, 59, 2494-2506 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Plasmodium falciparum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H1484Y | the mutant shows reduced activity compared to the wild type enzyme | Plasmodium falciparum |
| S1320L | the mutant shows reduced activity compared to the wild type enzyme | Plasmodium falciparum |
| Y1356F | the mutant shows reduced activity compared to the wild type enzyme | Plasmodium falciparum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| BQR695 | - |
Plasmodium falciparum |  |
| KDU691 | complete inhibition at 0.01 mM | Plasmodium falciparum | |
| MMV390048 | complete inhibition at 0.01 mM | Plasmodium falciparum | |
| NVP-BGT226 | complete inhibition at 0.01 mM | Plasmodium falciparum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0791 | - |
ATP | at pH 7.4 and 37°C | Plasmodium falciparum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 10 mM used in assay conditions | Plasmodium falciparum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1-phosphatidyl-1D-myo-inositol | Plasmodium falciparum | - |
ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | Q8I406 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HIS-Select nickel affinity gel chromatography | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1-phosphatidyl-1D-myo-inositol | - |
Plasmodium falciparum | ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Plasmodium falciparum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phosphatidylinositol 4-kinase IIIbeta | - |
Plasmodium falciparum |
| PI4KIIIbeta | - |
Plasmodium falciparum |
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