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Literature summary for 2.7.1.67 extracted from

  • Steinert, P.; Wissing, J.B.; Wagner, K.G.
    Manganese-stimulated phosphatidylinositol 4-kinase from Dunaliella parva: purification and characterization (1994), Plant Sci., 101, 105-114.
No PubMed abstract available
Search for more literature for 2.7.1.67

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 activity depends on presence of a surfactant. Optimal concentration is 3.7 mM Dunaliella parva

Inhibitors

Inhibitors Comment Organism Structure
adenosine
-
 Dunaliella parva
ADP
-
 Dunaliella parva
AMP
-
 Dunaliella parva
cAMP
-
 Dunaliella parva
CDP
-
 Dunaliella parva
CTP
-
 Dunaliella parva
GTP
-
 Dunaliella parva
phosphatidylethanolamine
-
 Dunaliella parva
UTP
-
 Dunaliella parva

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
 phosphatidylinositol pH 7.8, 28°C, at 0.75 mM Triton X-100 Dunaliella parva
0.055
-
 phosphatidylinositol pH 7.8, 28°C, at 1.5 mM Triton X-100 Dunaliella parva
0.39
-
 ATP pH 7.8, 28°C, at 2 mM MgCl2 and 10 mM MnCl2 Dunaliella parva
1.7
-
 ATP pH 7.8, 28°C, at 30 mM MgCl2 Dunaliella parva

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Dunaliella parva
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ enzyme requires divalent cations, Mg2+ with an optimal concentration of 30 mM, Ca2+ with an optimal concentration of 10 mM or Mn2+ with an optimal concentration of 10 mM. Mn2+ is the best activator. Mn2+, Mg2+ or Ca2+ activate synergistically Dunaliella parva
Mg2+ enzyme requires divalent cations, Mg2+ with an optimal concentration of 30 mM, Ca2+ with an optimal concentration of 10 mM or Mn2+ with an optimal concentration of 10 mM. Mn2+ is the best activator. Mn2+, Mg2+ or Ca2+ activate synergistically Dunaliella parva
Mn2+ enzyme requires divalent cations, Mg2+ with an optimal concentration of 30 mM, Ca2+ with an optimal concentration of 10 mM or Mn2+ with an optimal concentration of 10 mM. Mn2+ is the best activator. Mn2+, Mg2+ or Ca2+ activate synergistically Dunaliella parva
NaCl enhances activity up to about 1.3fold at 1 M Dunaliella parva

Organism

Organism UniProt Comment Textmining
Dunaliella parva
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Dunaliella parva

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.077
-
-
 Dunaliella parva

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol
-
 Dunaliella parva ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 at 30 mM MgCl2 Dunaliella parva
42
-
 at 2 mM MgCl2 and 10 mM MnCl2 Dunaliella parva

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Dunaliella parva

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.025
-
 ADP pH 7.8, 28°C Dunaliella parva
0.775
-
 adenosine pH 7.8, 28°C Dunaliella parva
1.2
-
 AMP pH 7.8, 28°C Dunaliella parva
1.68
-
 CTP pH 7.8, 28°C Dunaliella parva
1.68
-
 cAMP pH 7.8, 28°C Dunaliella parva
1.75
-
 GTP pH 7.8, 28°C Dunaliella parva
2.9
-
 CDP pH 7.8, 28°C Dunaliella parva
3.6
-
 UTP pH 7.8, 28°C Dunaliella parva

pI Value

Organism Comment pI Value Maximum pI Value
Dunaliella parva chromatofocusing
-
 5.5