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Literature summary for 2.7.1.64 extracted from

  • Sato, T.; Fujihashi, M.; Miyamoto, Y.; Kuwata, K.; Kusaka, E.; Fujita, H.; Miki, K.; Atomi, H.
    An uncharacterized member of the ribokinase family in Thermococcus kodakarensis exhibits myo-inositol kinase activity (2013), J. Biol. Chem., 288, 20856-20867.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL and Rosetta2(DE3)pLysS cells Thermococcus kodakarensis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, usingv 18-23% (w/v) polyethylene glycol 3350 and 0.2 M NH4I Thermococcus kodakarensis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 49% residual activity at 3 mM Thermococcus kodakarensis
EDTA
-
Thermococcus kodakarensis
Mn2+ 21% residual activity at 3 mM Thermococcus kodakarensis
Ni2+ 53% residual activity at 3 mM Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.36
-
myo-inositol at pH 7.5 and 85°C Thermococcus kodakarensis
53
-
D-tagatose at pH 7.5 and 85°C Thermococcus kodakarensis
171
-
D-Lyxose at pH 7.5 and 85°C Thermococcus kodakarensis
418
-
D-xylose at pH 7.5 and 85°C Thermococcus kodakarensis
438
-
D-talose at pH 7.5 and 85°C Thermococcus kodakarensis
524
-
D-xylulose at pH 7.5 and 85°C Thermococcus kodakarensis
863
-
D-allose at pH 7.5 and 85°C Thermococcus kodakarensis
951
-
D-ribose at pH 7.5 and 85°C Thermococcus kodakarensis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 133% activity at 3 mM Thermococcus kodakarensis
Zn2+ 125% activity at 3 mM Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30300
-
-
Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + myo-inositol Thermococcus kodakarensis
-
ADP + 1D-myo-inositol 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q5JDA3
-
-

Purification (Commentary)

Purification (Comment) Organism
Resource Q column chromatography, Resource PHE hydrophobic column chromatography, and Superdex 200 gel filtration Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.063
-
with meso-erythritol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
0.098
-
with L-arabitol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
0.17
-
with cis-inositol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
0.24
-
with L-chiro-inositol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
0.39
-
with adonitol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
0.5
-
with D-arabitol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
1.06
-
with myo-inositol 1-phosphate as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
1.91
-
with allo-inositol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
17.64
-
with scyllo-inositol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
34.92
-
with D-chiro-inositol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis
44.27
-
with myo-inositol as substrate, at pH 7.5 and 85°C Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-allose
-
Thermococcus kodakarensis ?
-
?
ATP + D-lyxose
-
Thermococcus kodakarensis ?
-
?
ATP + D-ribose
-
Thermococcus kodakarensis ?
-
?
ATP + D-tagatose
-
Thermococcus kodakarensis ?
-
?
ATP + D-talose
-
Thermococcus kodakarensis ?
-
?
ATP + D-xylose
-
Thermococcus kodakarensis ?
-
?
ATP + D-xylulose
-
Thermococcus kodakarensis ?
-
?
ATP + myo-inositol
-
Thermococcus kodakarensis ADP + 1D-myo-inositol 3-phosphate
-
?
additional information no activity with glycerol, DL-threitol, L-iditol, dulcitol, volemitol, D-lactitol monohydrate, maltitol, D-glucosamine, N-acetyl-D-glucosamine, maltose and lactose, D-erythrose, D-threose, D-erythrulose, L-arabinose, L-fructose, L-glucose, D-ribulose, D-galactose, fructose 1-phosphate, fructose 6-phosphate, glucose 1-phosphate, glucose 6-phosphate, ribose 1-phosphate, ribose 5-phosphate, tagatose 6-phosphate, adenosine, uridine, guanosine, cytidine, thymidine, and inosine Thermococcus kodakarensis ?
-
?

Subunits

Subunits Comment Organism
? x * 30300, calculated from amino acid sequence Thermococcus kodakarensis

Synonyms

Synonyms Comment Organism
myo-inositol kinase
-
Thermococcus kodakarensis
TK2285 protein
-
Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65 95 the enzyme is extremely thermostable with half-lives of 3 h at 95°C, 19 h at 85°C, 40 h at 75°C, and 88 h at 65°C Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
D-tagatose at pH 7.5 and 85°C Thermococcus kodakarensis
3.9
-
D-Lyxose at pH 7.5 and 85°C Thermococcus kodakarensis
5.1
-
D-ribose at pH 7.5 and 85°C Thermococcus kodakarensis
5.6
-
D-xylose at pH 7.5 and 85°C Thermococcus kodakarensis
11.6
-
D-xylulose at pH 7.5 and 85°C Thermococcus kodakarensis
13.1
-
D-talose at pH 7.5 and 85°C Thermococcus kodakarensis
18.3
-
D-allose at pH 7.5 and 85°C Thermococcus kodakarensis
23.2
-
myo-inositol at pH 7.5 and 85°C Thermococcus kodakarensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.005
-
D-ribose at pH 7.5 and 85°C Thermococcus kodakarensis
0.013
-
D-xylose at pH 7.5 and 85°C Thermococcus kodakarensis
0.021
-
D-allose at pH 7.5 and 85°C Thermococcus kodakarensis
0.022
-
D-xylulose at pH 7.5 and 85°C Thermococcus kodakarensis
0.023
-
D-Lyxose at pH 7.5 and 85°C Thermococcus kodakarensis
0.028
-
D-tagatose at pH 7.5 and 85°C Thermococcus kodakarensis
0.03
-
D-talose at pH 7.5 and 85°C Thermococcus kodakarensis
64.9
-
myo-inositol at pH 7.5 and 85°C Thermococcus kodakarensis