Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL and Rosetta2(DE3)pLysS cells | Thermococcus kodakarensis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, usingv 18-23% (w/v) polyethylene glycol 3350 and 0.2 M NH4I | Thermococcus kodakarensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 49% residual activity at 3 mM | Thermococcus kodakarensis | |
EDTA | - |
Thermococcus kodakarensis | |
Mn2+ | 21% residual activity at 3 mM | Thermococcus kodakarensis | |
Ni2+ | 53% residual activity at 3 mM | Thermococcus kodakarensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
myo-inositol | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
53 | - |
D-tagatose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
171 | - |
D-Lyxose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
418 | - |
D-xylose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
438 | - |
D-talose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
524 | - |
D-xylulose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
863 | - |
D-allose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
951 | - |
D-ribose | at pH 7.5 and 85°C | Thermococcus kodakarensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 133% activity at 3 mM | Thermococcus kodakarensis | |
Zn2+ | 125% activity at 3 mM | Thermococcus kodakarensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30300 | - |
- |
Thermococcus kodakarensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myo-inositol | Thermococcus kodakarensis | - |
ADP + 1D-myo-inositol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | Q5JDA3 | - |
- |
Purification (Comment) | Organism |
---|---|
Resource Q column chromatography, Resource PHE hydrophobic column chromatography, and Superdex 200 gel filtration | Thermococcus kodakarensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.063 | - |
with meso-erythritol as substrate, at pH 7.5 and 85°C | Thermococcus kodakarensis |
0.098 | - |
with L-arabitol as substrate, at pH 7.5 and 85°C | Thermococcus kodakarensis |
0.17 | - |
with cis-inositol as substrate, at pH 7.5 and 85°C | Thermococcus kodakarensis |
0.24 | - |
with L-chiro-inositol as substrate, at pH 7.5 and 85°C | Thermococcus kodakarensis |
0.39 | - |
with adonitol as substrate, at pH 7.5 and 85°C | Thermococcus kodakarensis |
0.5 | - |
with D-arabitol as substrate, at pH 7.5 and 85°C | Thermococcus kodakarensis |
1.06 | - |
with myo-inositol 1-phosphate as substrate, at pH 7.5 and 85°C | Thermococcus kodakarensis |
1.91 | - |
with allo-inositol as substrate, at pH 7.5 and 85°C | Thermococcus kodakarensis |
17.64 | - |
with scyllo-inositol as substrate, at pH 7.5 and 85°C | Thermococcus kodakarensis |
34.92 | - |
with D-chiro-inositol as substrate, at pH 7.5 and 85°C | Thermococcus kodakarensis |
44.27 | - |
with myo-inositol as substrate, at pH 7.5 and 85°C | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-allose | - |
Thermococcus kodakarensis | ? | - |
? | |
ATP + D-lyxose | - |
Thermococcus kodakarensis | ? | - |
? | |
ATP + D-ribose | - |
Thermococcus kodakarensis | ? | - |
? | |
ATP + D-tagatose | - |
Thermococcus kodakarensis | ? | - |
? | |
ATP + D-talose | - |
Thermococcus kodakarensis | ? | - |
? | |
ATP + D-xylose | - |
Thermococcus kodakarensis | ? | - |
? | |
ATP + D-xylulose | - |
Thermococcus kodakarensis | ? | - |
? | |
ATP + myo-inositol | - |
Thermococcus kodakarensis | ADP + 1D-myo-inositol 3-phosphate | - |
? | |
additional information | no activity with glycerol, DL-threitol, L-iditol, dulcitol, volemitol, D-lactitol monohydrate, maltitol, D-glucosamine, N-acetyl-D-glucosamine, maltose and lactose, D-erythrose, D-threose, D-erythrulose, L-arabinose, L-fructose, L-glucose, D-ribulose, D-galactose, fructose 1-phosphate, fructose 6-phosphate, glucose 1-phosphate, glucose 6-phosphate, ribose 1-phosphate, ribose 5-phosphate, tagatose 6-phosphate, adenosine, uridine, guanosine, cytidine, thymidine, and inosine | Thermococcus kodakarensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30300, calculated from amino acid sequence | Thermococcus kodakarensis |
Synonyms | Comment | Organism |
---|---|---|
myo-inositol kinase | - |
Thermococcus kodakarensis |
TK2285 protein | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 95 | the enzyme is extremely thermostable with half-lives of 3 h at 95°C, 19 h at 85°C, 40 h at 75°C, and 88 h at 65°C | Thermococcus kodakarensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
D-tagatose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
3.9 | - |
D-Lyxose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
5.1 | - |
D-ribose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
5.6 | - |
D-xylose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
11.6 | - |
D-xylulose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
13.1 | - |
D-talose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
18.3 | - |
D-allose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
23.2 | - |
myo-inositol | at pH 7.5 and 85°C | Thermococcus kodakarensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
D-ribose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
0.013 | - |
D-xylose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
0.021 | - |
D-allose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
0.022 | - |
D-xylulose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
0.023 | - |
D-Lyxose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
0.028 | - |
D-tagatose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
0.03 | - |
D-talose | at pH 7.5 and 85°C | Thermococcus kodakarensis | |
64.9 | - |
myo-inositol | at pH 7.5 and 85°C | Thermococcus kodakarensis |