Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.63 extracted from

  • Girbal, E.; Binot, R.A.; Monsan, P.F.
    Production, purification, properties and kinetic studies of free and immobilized polyphosphate:glucose-6-phosphotransferase from Mycobacterium phlei (1989), Enzyme Microb. Technol., 11, 518-527.
No PubMed abstract available
Search for more literature for 2.7.1.63

General Stability

General Stability Organism
dialysed enzyme preparations are largely inactive, Mg2+ restores activity Mycolicibacterium phlei
enzyme immobilized on corn stover is stable for one months at pH 8.5, 30°C Mycolicibacterium phlei

Inhibitors

Inhibitors Comment Organism Structure
D-glucose above 150 mM, substrate inhibition Mycolicibacterium phlei
Mg2+ molar ratio polyphosphate/Mg below 0.5, activation above, EDTA partially reverses Mycolicibacterium phlei
Poly(P) above 0.05 mM, substrate inhibition Mycolicibacterium phlei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0072
-
 (phosphate)15 pH 8.5, 30°C, soluble enzyme Mycolicibacterium phlei
0.13
-
 (phosphate)15 pH 8.5, 30°C, immobilized enzyme Mycolicibacterium phlei
0.48
-
 D-glucose pH 8.5, 30°C, soluble enzyme Mycolicibacterium phlei
0.6
-
 D-glucose pH 8.5, 30°C, immobilized enzyme Mycolicibacterium phlei

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Mycolicibacterium phlei 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation, about 30% as efficient as Mg2+ Mycolicibacterium phlei
Mg2+ activation Mycolicibacterium phlei
Mg2+ molar ratio poly(P)/Mg2+ above 0.5 inhibition below Mycolicibacterium phlei
Mn2+ activation, as effective as Mg2+ Mycolicibacterium phlei
Zn2+ activation, about 30% as efficient as Mg2+ Mycolicibacterium phlei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 x * 34000, SDS-PAGE Mycolicibacterium phlei
275000 280000 non-denaturing PAGE, gel filtration Mycolicibacterium phlei

Organism

Organism UniProt Comment Textmining
Mycobacterium gordonae
-
-
-
Mycolicibacterium aurum
-
-
-
Mycolicibacterium phlei
-
-
-
Mycolicibacterium smegmatis
-
-
-
Mycolicibacterium thermoresistibile
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycolicibacterium phlei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
140
-
-
 Mycolicibacterium phlei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(phosphate)15 + D-glucose
-
 Mycolicibacterium phlei (phosphate)14 + D-glucose 6-phosphate
-
 ?
(phosphate)n + D-glucose
-
 Mycolicibacterium smegmatis (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
(phosphate)n + D-glucose
-
 Mycolicibacterium aurum (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
(phosphate)n + D-glucose
-
 Mycobacterium gordonae (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
(phosphate)n + D-glucose
-
 Mycolicibacterium thermoresistibile (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
(phosphate)n + D-glucose i.e. poly(P)n, no substrates are monophosphate, diphosphate or triphosphate Mycolicibacterium phlei (phosphate)n-1 + D-glucose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Mycolicibacterium phlei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4 8.3 broad plateau Mycolicibacterium phlei

pH Range

pH Minimum pH Maximum Comment Organism
6 8.4 very little activity below pH 6.0 and above pH 8.4 Mycolicibacterium phlei

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.1 8.4 immobilized enzyme is stable Mycolicibacterium phlei

pI Value

Organism Comment pI Value Maximum pI Value
Mycolicibacterium phlei isoelectric focusing
-
 5