Literature summary for 2.7.1.60 extracted from

Caing-Carlsson, R.; Goyal, P.; Sharma, A.; Ghosh, S.; Setty, T.G.; North, R.A.; Friemann, R.; Ramaswamy, S.
Crystal structure of N-acetylmannosamine kinase from Fusobacterium nucleatum (2017), Acta Crystallogr. Sect. F, 73, 356-362 .

Search for more literature for 2.7.1.60

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Fusobacterium nucleatum subsp. nucleatum

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 0.2 M lithium sulfate monohydrate, 0.1 M Tris-HCl pH 8.5, 30% (w/v) PEG 4000	Fusobacterium nucleatum subsp. nucleatum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Fusobacterium nucleatum subsp. nucleatum	5737	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + N-acyl-D-mannosamine	Fusobacterium nucleatum subsp. nucleatum	-	ADP + N-acyl-D-mannosamine 6-phosphate	-	?
ATP + N-acyl-D-mannosamine	Fusobacterium nucleatum subsp. nucleatum DSM 15643	-	ADP + N-acyl-D-mannosamine 6-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Fusobacterium nucleatum subsp. nucleatum	Q8RDN7	-	-
Fusobacterium nucleatum subsp. nucleatum DSM 15643	Q8RDN7	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography and Superdex S200 gel filtration	Fusobacterium nucleatum subsp. nucleatum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + N-acyl-D-mannosamine	-	Fusobacterium nucleatum subsp. nucleatum	ADP + N-acyl-D-mannosamine 6-phosphate	-	?
ATP + N-acyl-D-mannosamine	-	Fusobacterium nucleatum subsp. nucleatum DSM 15643	ADP + N-acyl-D-mannosamine 6-phosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Fusobacterium nucleatum subsp. nucleatum

Synonyms

Synonyms	Comment	Organism
N-acetylmannosamine kinase	-	Fusobacterium nucleatum subsp. nucleatum
nanK	-	Fusobacterium nucleatum subsp. nucleatum

General Information

General Information	Comment	Organism
metabolism	the enzyme catalyzes the second step of the sialic acid catabolic pathway	Fusobacterium nucleatum subsp. nucleatum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)