Cloned (Comment) | Organism |
---|---|
expressed as His-tag fusion protein in Sf9, and Sf-900 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-234 | monomer molecular mass of 58000 Da, complete loss of epimerase activity, 90% loss of kinase activity | Rattus norvegicus |
DELTA1-359 | monomer molecular mass of 45000 Da, complete loss of epimerase activity, almost complete loss of kinase activity | Rattus norvegicus |
DELTA1-39 | monomer molecular mass of 79000 Da, complete loss of epimerase activity, 75% loss of kinase activity | Rattus norvegicus |
DELTA383-722 | monomer molecular mass of 49000 Da, almost complete loss of epimerase activity, complete loss of kinase activity | Rattus norvegicus |
DELTA490-722 | monomer molecular mass of 61000 Da, more than 80% loss of epimerase activity, complete loss of kinase activity | Rattus norvegicus |
DELTA597-722 | monomer molecular mass of 72000 Da, almost complete loss of epimerase activity, and kinase activity | Rattus norvegicus |
DELTA697-722 | monomer molecular mass of 83000 Da, 25% loss of epimerase activity, 60% loss of kinase activity | Rattus norvegicus |
DELTA717-722 | monomer molecular mass of 85000 Da, less than 10% loss of epimerase activity, 20% loss of kinase activity | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79000 | - |
6 * 79000, His-tagged protein has a mass of 85000 Da, SDS-PAGE, native mass by gel filtration | Rattus norvegicus |
500000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acyl-D-mannosamine | Rattus norvegicus | key enzyme in the synthesis of N-acetylneuraminic acid and therefore of nearly all other sialic acids | ADP + N-acyl-D-mannosamine 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acyl-D-mannosamine | - |
Rattus norvegicus | ADP + N-acyl-D-mannosamine 6-phosphate | - |
? | |
ATP + N-acyl-D-mannosamine | key enzyme in the synthesis of N-acetylneuraminic acid and therefore of nearly all other sialic acids | Rattus norvegicus | ADP + N-acyl-D-mannosamine 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 79000, His-tagged protein has a mass of 85000 Da, SDS-PAGE, native mass by gel filtration | Rattus norvegicus |
trimer | some of the deletion mutants tends to form trimers | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
UDP-GlcNAc 2-epimerase/ManNAc kinase | - |
Rattus norvegicus |
UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase | two different enzyme activities combined in a single bifunctional enzyme, N-terminal region is for epimerase activity, C-terminal region is for kinase activity | Rattus norvegicus |