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Literature summary for 2.7.1.60 extracted from

  • Hinderlich, S.; Stäsche, R.; Zeitler, R.; Reutter, W.
    A bifunctional enzyme catalyzes the first two steps in N-acetylneuraminic acid biosynthesis of rat liver. Purification and characterization of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (1997), J. Biol. Chem., 272, 24313-24318.
    View publication on PubMed
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General Stability

General Stability Organism
hexameric enzyme is completely stable with 0.1 mM UDP Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.093
-
 N-acetyl-D-mannosamine pH 7.5, 37°C, hexamer Rattus norvegicus
0.121
-
 N-acetyl-D-mannosamine pH 7.5, 37°C, dimer Rattus norvegicus
1.18
-
 ATP pH 7.5, 37°C, hexamer Rattus norvegicus
1.67
-
 ATP pH 7.5, 37°C, dimer Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 gel filtration, SDS-PAGE Rattus norvegicus
150000
-
 gel filtration, ion of the monomeric 75000 Da protein self associate as dimer Rattus norvegicus
450000
-
 gel filtration, ion of the monomeric 75000 Da protein self associate as hexamer Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acetyl-D-mannosamine Rattus norvegicus
-
 ADP + N-acetyl-D-mannosamine 6-phosphate
-
 ?
ATP + N-acetyl-D-mannosamine Rattus norvegicus Wistar
-
 ADP + N-acetyl-D-mannosamine 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Wistar
-
Rattus norvegicus Wistar
-
 Wistar
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
intestinal mucosa
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-
salivary gland
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.114
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-70°C, no loss of activity observed for several months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-D-mannosamine
-
 Rattus norvegicus ADP + N-acetyl-D-mannosamine 6-phosphate
-
 ?
ATP + N-acetyl-D-mannosamine
-
 Rattus norvegicus Wistar ADP + N-acetyl-D-mannosamine 6-phosphate
-
 ?
ATP + N-acetyl-D-mannosamine
-
 Rattus norvegicus ADP + N-acetyl-D-mannosamine-6-phosphate
-
 ?
ATP + N-acetyl-D-mannosamine
-
 Rattus norvegicus Wistar ADP + N-acetyl-D-mannosamine-6-phosphate
-
 ?
additional information UDP-GlcNAc 2-epimerase and ManNAc kinase are parts of one bifunctional enzyme, catalyzes the first 2 steps in N-acetylneuraminic acid biosynthesis Rattus norvegicus ?
-
 ?
additional information UDP-GlcNAc 2-epimerase and ManNAc kinase are parts of one bifunctional enzyme, catalyzes the first 2 steps in N-acetylneuraminic acid biosynthesis Rattus norvegicus Wistar ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 75000, self associates as a dimer, gel filtration, SDS-PAGE, catalyzes only phosphorylation of N-acetylmannosamine, incubation with UDP-N-acetylglucosamine leads to reassembly of the fully active hexamer Rattus norvegicus
hexamer 6 * 75000, self associates as a hexamer, gel filtration, SDS-PAGE, catalyzes both enzyme activities, UDP-GlcNAc-epimerase/ManNAc kinase Rattus norvegicus

Synonyms

Synonyms Comment Organism
ManAc kinase
-
 Rattus norvegicus
UDP-GlcNAc-epimerase/ManNAc kinase
-
 Rattus norvegicus