Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli HMS174(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E174Q | no detectable activity, less stable enzyme | Homo sapiens |
R105M | modest reduction in activity with little change in stability | Homo sapiens |
R228M | interaction with both ATP and galactose is affected. The variant is significantly less stable than the wild-type protein | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
ATP | mutant enzyme E174D, pH 7.5, 37°C | Homo sapiens | |
0.004 | - |
ATP | wild-type enzyme, pH 7.5, 37°C | Homo sapiens | |
0.0094 | - |
ATP | mutant enzyme R228M, pH 7.5, 37°C | Homo sapiens | |
0.0149 | - |
ATP | mutant enzyme R228K, pH 7.5, 37°C | Homo sapiens | |
0.083 | - |
ATP | mutant enzyme R105M, pH 7.5, 37°C | Homo sapiens | |
0.22 | - |
alpha-D-galactose | mutant enzyme E174D, pH 7.5, 37°C | Homo sapiens | |
0.26 | - |
alpha-D-galactose | mutant enzyme R105M, pH 7.5, 37°C | Homo sapiens | |
0.31 | - |
alpha-D-galactose | mutant enzyme R228K, pH 7.5, 37°C | Homo sapiens | |
0.33 | - |
alpha-D-galactose | wild-type enzyme, pH 7.5, 37°C | Homo sapiens | |
0.67 | - |
alpha-D-galactose | mutant enzyme R228M, pH 7.5, 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-galactose | Homo sapiens | the enzyme catalyses the first committed step in the Leloir pathway | ADP + alpha-D-galactose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P51570 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-galactose | the enzyme catalyses the first committed step in the Leloir pathway | Homo sapiens | ADP + alpha-D-galactose 1-phosphate | - |
? | |
ATP + alpha-D-galactose | negative charge at residue 174 is essential for catalysis | Homo sapiens | ADP + alpha-D-galactose 1-phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
51.3 | - |
Tm-value, mutant enzyme R228K | Homo sapiens |
51.68 | - |
Tm-value, mutant enzyme R228M | Homo sapiens |
52.5 | - |
Tm-value, mutant enzyme E174Q | Homo sapiens |
55.13 | - |
Tm-value, mutant enzyme E174D | Homo sapiens |
55.42 | - |
Tm-value, mutant enzyme R105M | Homo sapiens |
55.72 | - |
Tm-value, wild-type enzyme | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
ATP | mutant enzyme R105M, pH 7.5, 37°C | Homo sapiens | |
0.7 | - |
alpha-D-galactose | mutant enzyme R105M, pH 7.5, 37°C | Homo sapiens | |
1.6 | - |
ATP | mutant enzyme E174D, pH 7.5, 37°C | Homo sapiens | |
1.6 | - |
alpha-D-galactose | mutant enzyme E174D, pH 7.5, 37°C | Homo sapiens | |
1.7 | - |
ATP | wild-type enzyme, pH 7.5, 37°C | Homo sapiens | |
1.7 | - |
alpha-D-galactose | wild-type enzyme, pH 7.5, 37°C | Homo sapiens | |
2.3 | - |
ATP | mutant enzyme R228M, pH 7.5, 37°C | Homo sapiens | |
2.3 | - |
alpha-D-galactose | mutant enzyme R228M, pH 7.5, 37°C | Homo sapiens | |
3.7 | - |
ATP | mutant enzyme R228K, pH 7.5, 37°C | Homo sapiens | |
3.7 | - |
alpha-D-galactose | mutant enzyme R228K, pH 7.5, 37°C | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
drug target | possible drug target in the treatment of type I galactosemia | Homo sapiens |
metabolism | the enzyme catalyses the first committed step in the Leloir pathway | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
alpha-D-galactose | mutant enzyme R105M, pH 7.5, 37°C | Homo sapiens | |
3.3 | - |
alpha-D-galactose | mutant enzyme R228M, pH 7.5, 37°C | Homo sapiens | |
7.6 | - |
alpha-D-galactose | mutant enzyme E174D, pH 7.5, 37°C | Homo sapiens | |
10.1 | - |
alpha-D-galactose | wild-type enzyme, pH 7.5, 37°C | Homo sapiens | |
19.6 | - |
alpha-D-galactose | mutant enzyme R228K, pH 7.5, 37°C | Homo sapiens | |
98 | - |
ATP | mutant enzyme R105M, pH 7.5, 37°C | Homo sapiens | |
240 | - |
ATP | mutant enzyme R228M, pH 7.5, 37°C | Homo sapiens | |
250 | - |
ATP | mutant enzyme R228K, pH 7.5, 37°C | Homo sapiens | |
340 | - |
ATP | mutant enzyme E174D, pH 7.5, 37°C | Homo sapiens | |
430 | - |
ATP | wild-type enzyme, pH 7.5, 37°C | Homo sapiens |