Any feedback?
Literature summary for 2.7.1.6 extracted from
- Mechanistic implications of Escherichia coli galactokinase structure-based engineering (2004), ChemBioChem, 5, 989-992.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | all exchanges for D37 are detrimental to the enzyme | Escherichia coli |
| Y223F | activity similar to wild-type, converts additional substrates L-glucose, D-talose, 4-deoxy-D-galactose, less active on D-galactosamine than wild-type | Escherichia coli |
| Y223H | significant reduction in activity | Escherichia coli |
| Y223L | significant reduction in activity | Escherichia coli |
| Y223R | significant reduction in activity | Escherichia coli |
| Y223W | significant reduction in activity, converts additional substrates L-glucose, D-talose, 4-deoxy-D-galactose, with less efficiency than mutant Y223F, less active on D-galactosamine than wild-type | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.78 | - |
ATP | mutant Y223W | Escherichia coli |  |
| 2.24 | - |
ATP | wild-type | Escherichia coli | |
| 3.58 | - |
ATP | mutant Y223F | Escherichia coli | |
| 4.46 | - |
D-galactose | mutant Y223F | Escherichia coli | |
| 5.2 | - |
D-galactose | wild-type | Escherichia coli | |
| 5.63 | - |
D-galactose | mutant Y223W | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.61 | - |
mutant Y223H | Escherichia coli |
| 0.63 | - |
mutant Y223R | Escherichia coli |
| 0.65 | - |
mutant Y223L | Escherichia coli |
| 0.87 | - |
mutant Y223W | Escherichia coli |
| 23.42 | - |
mutant Y223F | Escherichia coli |
| 23.5 | - |
wild-type | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-deoxy-D-galactose | - |
Escherichia coli | ADP + 2-deoxy-alpha-D-galactose 1-phosphate | - |
? | |
| ATP + 4-deoxy-D-galactose | substrate only for mutants Y223F, Y223W | Escherichia coli | ADP + 4-deoxy-alpha-D-galactose 1-phosphate | - |
? | |
| ATP + 6-deoxy-D-galactose | - |
Escherichia coli | ADP + 6-deoxy-alpha-D-galactose 1-phosphate | - |
? | |
| ATP + D-galactosamine | - |
Escherichia coli | ADP + D-galactosamine 1-phosphate | - |
? | |
| ATP + D-galactose | - |
Escherichia coli | ADP + alpha-D-galactose 1-phosphate | - |
? | |
| ATP + D-talose | substrate only for mutants Y223F, Y223W | Escherichia coli | ADP + alpha-D-talose 1-phosphate | - |
? | |
| ATP + L-glucose | substrate only for mutants Y223F, Y223W | Escherichia coli | ADP + alpha-L-glucose 1-phosphate | - |
? | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
