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Literature summary for 2.7.1.59 extracted from

  • Rao, K.H.; Ghosh, S.; Natarajan, K.; Datta, A.
    N-acetylglucosamine kinase, HXK1 is involved in morphogenetic transition and metabolic gene expression in Candida albicans (2013), PLoS ONE, 8, e53638.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.59

Cloned(Commentary)

Cloned (Comment) Organism
gene hxk1, expression of GFP-tagged wild-type enzyme in enzyme-deficient disruption mutant Candida albicans strain CAI4 and expression of TAP- and His-tagged HXK1 under ADH1 p in CAI-4, co-expression with histone deacetylase SIR2 Candida albicans

Protein Variants

Protein Variants Comment Organism
additional information construction of a homozygous hxk1 mutant H8-1-103 Candida albicans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Candida albicans 5737
-
mitochondrion
-
 Candida albicans 5739
-
nucleus
-
 Candida albicans 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acetyl-D-glucosamine Candida albicans
-
 ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
additional information Candida albicans interaction of HXK1 with histone deacetylase SIR2 under filamentation inducing conditions ?
-
 ?

Organism

Organism UniProt Comment Textmining
Candida albicans
-
 gene hxk1
-

Purification (Commentary)

Purification (Comment) Organism
recombinnat TAP- and His-tagged HXK1 by tandem affinity purification using anti-FLAG and Ni-nitrilotriacetic acid agarose Candida albicans

Source Tissue

Source Tissue Comment Organism Textmining
additional information HXK1 expression levels are upregulated in filamentation inducing media, morphological and expression pattern analyses among wild type, hxk1 and different filamentation pathway mutants, overview Candida albicans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-D-glucosamine
-
 Candida albicans ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
additional information interaction of HXK1 with histone deacetylase SIR2 under filamentation inducing conditions Candida albicans ?
-
 ?

Synonyms

Synonyms Comment Organism
Hxk1
-
 Candida albicans

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Candida albicans

Expression

Organism Comment Expression
Candida albicans HXK1 expression levels are upregulated in filamentation inducing media up

General Information

General Information Comment Organism
malfunction the homozygous hxk1 mutant H8-1-103 is constitutively filamentous and hyperfilamentous under filamentation inducing conditions Candida albicans
metabolism HXK1 acts as both positive and negative regulator of transcription of genes involved in maintaining cellular homeostasis, regulatory mechanism involved in filamentation repression, overview Candida albicans
physiological function HXK1 plays a crucial role in morphogenesis, GlcNAc signaling/metabolic genes expression and maintenance of various cellular functions in Candida albicans, Hxk1 is involved in the cell wall synthesis and in repression of hyphal specific genes in addition to metabolic genes. Its regulation of filamentation and GlcNAc metabolism is independent of the known classical regulators like EFG1, CPH1, RAS1, TPK2 or TUP1, regulatory mechanism involved in filamentation repression, overview Candida albicans