Cloned (Comment) | Organism |
---|---|
gene hxk1, expression of GFP-tagged wild-type enzyme in enzyme-deficient disruption mutant Candida albicans strain CAI4 and expression of TAP- and His-tagged HXK1 under ADH1 p in CAI-4, co-expression with histone deacetylase SIR2 | Candida albicans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a homozygous hxk1 mutant H8-1-103 | Candida albicans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Candida albicans | 5737 | - |
mitochondrion | - |
Candida albicans | 5739 | - |
nucleus | - |
Candida albicans | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Candida albicans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-D-glucosamine | Candida albicans | - |
ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
additional information | Candida albicans | interaction of HXK1 with histone deacetylase SIR2 under filamentation inducing conditions | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
gene hxk1 | - |
Purification (Comment) | Organism |
---|---|
recombinnat TAP- and His-tagged HXK1 by tandem affinity purification using anti-FLAG and Ni-nitrilotriacetic acid agarose | Candida albicans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | HXK1 expression levels are upregulated in filamentation inducing media, morphological and expression pattern analyses among wild type, hxk1 and different filamentation pathway mutants, overview | Candida albicans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-D-glucosamine | - |
Candida albicans | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
additional information | interaction of HXK1 with histone deacetylase SIR2 under filamentation inducing conditions | Candida albicans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Hxk1 | - |
Candida albicans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Candida albicans |
Organism | Comment | Expression |
---|---|---|
Candida albicans | HXK1 expression levels are upregulated in filamentation inducing media | up |
General Information | Comment | Organism |
---|---|---|
malfunction | the homozygous hxk1 mutant H8-1-103 is constitutively filamentous and hyperfilamentous under filamentation inducing conditions | Candida albicans |
metabolism | HXK1 acts as both positive and negative regulator of transcription of genes involved in maintaining cellular homeostasis, regulatory mechanism involved in filamentation repression, overview | Candida albicans |
physiological function | HXK1 plays a crucial role in morphogenesis, GlcNAc signaling/metabolic genes expression and maintenance of various cellular functions in Candida albicans, Hxk1 is involved in the cell wall synthesis and in repression of hyphal specific genes in addition to metabolic genes. Its regulation of filamentation and GlcNAc metabolism is independent of the known classical regulators like EFG1, CPH1, RAS1, TPK2 or TUP1, regulatory mechanism involved in filamentation repression, overview | Candida albicans |