General Stability | Organism |
---|---|
freeze-thawing, stable to | Clostridium pasteurianum |
Tris-HCl buffer in purification and electrophoresis leads to partial denaturation and loss of activity, stable in phosphate buffer | Clostridium pasteurianum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | competitive vs. ATP | Clostridium pasteurianum | |
ATP | - |
Clostridium pasteurianum | |
CTP | if concentration exceeds Mg2+ concentration | Clostridium pasteurianum | |
Cu2+ | 0.5 mM, 50% inhibition in the presence of 3 mM Mg2+ | Clostridium pasteurianum | |
D-fructose 1,6-bisphosphate | noncompetitive vs. fructose 1-phosphate | Clostridium pasteurianum | |
GTP | - |
Clostridium pasteurianum | |
Hg2+ | 0.002 mM, 50% inhibition in the presence of 3 mM Mg2+ | Clostridium pasteurianum | |
ITP | - |
Clostridium pasteurianum | |
additional information | not inhibited by cAMP, pyruvate, phosphoenolpyruvate; not inhibited by citrate; not inhibited by D-fructose 6-phosphate; not inhibited by D-glucose 1-phosphate; not inhibited by D-glucose 6-phosphate; not inhibited by monovalent cations, glucose, ADPglucose, UDPglucose, NAD(H), NADP(H), dihydroxyacetone phosphate, glyceraldehyde 3-phosphate, 3-phosphoglycerate, CoA, acetyl-CoA, acetoacetyl-CoA; not inhibited by phosphate, AMP | Clostridium pasteurianum | |
Ni2+ | 3 mM, 50% inhibition in the presence of 3 mM Mg2+ | Clostridium pasteurianum | |
SO42- | - |
Clostridium pasteurianum | |
UTP | if concentration exceeds Mg2+ concentration | Clostridium pasteurianum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
ATP | pH 8.0, 25°C | Clostridium pasteurianum | |
0.62 | - |
D-Fructose 1-phosphate | pH 8.0, 25°C | Clostridium pasteurianum | |
0.67 | - |
ITP | pH 8.0, 25°C | Clostridium pasteurianum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 3 mM, 80% of activity with Mn2+ | Clostridium pasteurianum | |
Fe2+ | 3 mM, 50% of activity with Mn2+ | Clostridium pasteurianum | |
Mg2+ | required for activity | Clostridium pasteurianum | |
Mg2+ | 3 mM, 84% of activity with Mn2+ | Clostridium pasteurianum | |
Mn2+ | can replace Mg2+ | Clostridium pasteurianum | |
Mn2+ | required for activity | Clostridium pasteurianum | |
Zn2+ | 3 mM, 34% of activity with Mn2+ | Clostridium pasteurianum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21400 | - |
1 * 21400 + 1 * 35500, SDS-PAGE, after treatment with 8 M urea and 2-mercaptoethanol | Clostridium pasteurianum |
28500 | - |
1 * 28500 + 1 * 40000, SDS-PAGE, after treatment with 2-mercaptoethanol | Clostridium pasteurianum |
35500 | - |
1 * 21400 + 1 * 35500, SDS-PAGE, after treatment with 8 M urea and 2-mercaptoethanol | Clostridium pasteurianum |
40000 | - |
1 * 28500 + 1 * 40000, SDS-PAGE, after treatment with 2-mercaptoethanol | Clostridium pasteurianum |
62500 | - |
low-speed sedimentation equilibrium | Clostridium pasteurianum |
63100 | - |
gel filtration | Clostridium pasteurianum |
63200 | - |
sucrose density gradient centrifugation | Clostridium pasteurianum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium pasteurianum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
CM-Sephadex, calcium phosphate gel, Sephadex G-100, DEAE-Sephadex, hydroxyapatite | Clostridium pasteurianum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Clostridium pasteurianum |
Storage Stability | Organism |
---|---|
-20°C, 50 mM phosphate buffer, pH 7.5, 1 year, no loss of activity | Clostridium pasteurianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 1-phosphate | - |
Clostridium pasteurianum | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ITP + D-fructose 1-phosphate | - |
Clostridium pasteurianum | IDP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 21400 + 1 * 35500, SDS-PAGE, after treatment with 8 M urea and 2-mercaptoethanol | Clostridium pasteurianum |
dimer | 1 * 28500 + 1 * 40000, SDS-PAGE, after treatment with 2-mercaptoethanol | Clostridium pasteurianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 9.2 | - |
Clostridium pasteurianum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.8 | approx. half-maximal activity at pH 7 and 9.8 | Clostridium pasteurianum |