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Literature summary for 2.7.1.56 extracted from

  • Van Hugo, H.; Gottschalk, G.
    Purification and properties of 1-phosphofructokinase from Clostridium pasteurianum (1974), Eur. J. Biochem., 48, 455-463.
    View publication on PubMed

General Stability

General Stability Organism
freeze-thawing, stable to Clostridium pasteurianum
Tris-HCl buffer in purification and electrophoresis leads to partial denaturation and loss of activity, stable in phosphate buffer Clostridium pasteurianum

Inhibitors

Inhibitors Comment Organism Structure
ADP competitive vs. ATP Clostridium pasteurianum
ATP
-
Clostridium pasteurianum
CTP if concentration exceeds Mg2+ concentration Clostridium pasteurianum
Cu2+ 0.5 mM, 50% inhibition in the presence of 3 mM Mg2+ Clostridium pasteurianum
D-fructose 1,6-bisphosphate noncompetitive vs. fructose 1-phosphate Clostridium pasteurianum
GTP
-
Clostridium pasteurianum
Hg2+ 0.002 mM, 50% inhibition in the presence of 3 mM Mg2+ Clostridium pasteurianum
ITP
-
Clostridium pasteurianum
additional information not inhibited by cAMP, pyruvate, phosphoenolpyruvate; not inhibited by citrate; not inhibited by D-fructose 6-phosphate; not inhibited by D-glucose 1-phosphate; not inhibited by D-glucose 6-phosphate; not inhibited by monovalent cations, glucose, ADPglucose, UDPglucose, NAD(H), NADP(H), dihydroxyacetone phosphate, glyceraldehyde 3-phosphate, 3-phosphoglycerate, CoA, acetyl-CoA, acetoacetyl-CoA; not inhibited by phosphate, AMP Clostridium pasteurianum
Ni2+ 3 mM, 50% inhibition in the presence of 3 mM Mg2+ Clostridium pasteurianum
SO42-
-
Clostridium pasteurianum
UTP if concentration exceeds Mg2+ concentration Clostridium pasteurianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
ATP pH 8.0, 25°C Clostridium pasteurianum
0.62
-
D-Fructose 1-phosphate pH 8.0, 25°C Clostridium pasteurianum
0.67
-
ITP pH 8.0, 25°C Clostridium pasteurianum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 3 mM, 80% of activity with Mn2+ Clostridium pasteurianum
Fe2+ 3 mM, 50% of activity with Mn2+ Clostridium pasteurianum
Mg2+ required for activity Clostridium pasteurianum
Mg2+ 3 mM, 84% of activity with Mn2+ Clostridium pasteurianum
Mn2+ can replace Mg2+ Clostridium pasteurianum
Mn2+ required for activity Clostridium pasteurianum
Zn2+ 3 mM, 34% of activity with Mn2+ Clostridium pasteurianum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21400
-
1 * 21400 + 1 * 35500, SDS-PAGE, after treatment with 8 M urea and 2-mercaptoethanol Clostridium pasteurianum
28500
-
1 * 28500 + 1 * 40000, SDS-PAGE, after treatment with 2-mercaptoethanol Clostridium pasteurianum
35500
-
1 * 21400 + 1 * 35500, SDS-PAGE, after treatment with 8 M urea and 2-mercaptoethanol Clostridium pasteurianum
40000
-
1 * 28500 + 1 * 40000, SDS-PAGE, after treatment with 2-mercaptoethanol Clostridium pasteurianum
62500
-
low-speed sedimentation equilibrium Clostridium pasteurianum
63100
-
gel filtration Clostridium pasteurianum
63200
-
sucrose density gradient centrifugation Clostridium pasteurianum

Organism

Organism UniProt Comment Textmining
Clostridium pasteurianum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
CM-Sephadex, calcium phosphate gel, Sephadex G-100, DEAE-Sephadex, hydroxyapatite Clostridium pasteurianum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Clostridium pasteurianum

Storage Stability

Storage Stability Organism
-20°C, 50 mM phosphate buffer, pH 7.5, 1 year, no loss of activity Clostridium pasteurianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 1-phosphate
-
Clostridium pasteurianum ADP + D-fructose 1,6-bisphosphate
-
?
ITP + D-fructose 1-phosphate
-
Clostridium pasteurianum IDP + D-fructose 1,6-bisphosphate
-
?

Subunits

Subunits Comment Organism
dimer 1 * 21400 + 1 * 35500, SDS-PAGE, after treatment with 8 M urea and 2-mercaptoethanol Clostridium pasteurianum
dimer 1 * 28500 + 1 * 40000, SDS-PAGE, after treatment with 2-mercaptoethanol Clostridium pasteurianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 9.2
-
Clostridium pasteurianum

pH Range

pH Minimum pH Maximum Comment Organism
7 9.8 approx. half-maximal activity at pH 7 and 9.8 Clostridium pasteurianum