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Literature summary for 2.7.1.52 extracted from

  • Liu, T.W.; Kaji, H.; Togayachi, A.; Ito, H.; Sato, T.; Narimatsu, H.
    A chemoenzymatic approach toward the identification of fucosylated glycoproteins and mapping of N-glycan sites (2012), Glycobiology, 22, 630-637.
    View publication on PubMed
Search for more literature for 2.7.1.52

Application

Application Comment Organism
analysis development of an alternative way of labeling of fucosylated structures by metabolic engineering for the identification of fucosylated glycoproteins, using a chemoenzymatic approach. In this approach, the activities of Bacteroides fragilis 9343 L-fucokinase/guanosine-5'-diphosphate-Fuc pyrophosphorylase and human alpha1,3-fucosyltransferase 9 are combined in a Namalwa cellular model, the system can be applied to labeling of alkyne-modified fucosylated glycoproteins, e.g. for labeling of alkynyl fucose containing glycans, N-linked glycosylation site mapping and fucosylated glycoprotein identification by IGOT-LC-MS analysis, overview Bacteroides fragilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bacteroides fragilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-fucose Bacteroides fragilis
-
 ADP + beta-L-fucose 1-phosphate
-
 ?
ATP + L-fucose Bacteroides fragilis 9343
-
 ADP + beta-L-fucose 1-phosphate
-
 ?
additional information Bacteroides fragilis development of an alternative way of labeling of fucosylated structures by metabolic engineering for the identification of fucosylated glycoproteins, using a chemoenzymatic approach. In this approach, the activities of Bacteroides fragilis 9343 L-fucokinase/guanosine-5'-diphosphate-Fuc pyrophosphorylase and human alpha1,3-fucosyltransferase 9 are combined in a Namalwa cellular model, the system can be applied to labeling of alkyne-modified fucosylated glycoproteins, e.g. for labeling of alkynyl fucose containing glycans, N-linked glycosylation site mapping and fucosylated glycoprotein identification by IGOT-LC-MS analysis, overview ?
-
 ?
additional information Bacteroides fragilis 9343 development of an alternative way of labeling of fucosylated structures by metabolic engineering for the identification of fucosylated glycoproteins, using a chemoenzymatic approach. In this approach, the activities of Bacteroides fragilis 9343 L-fucokinase/guanosine-5'-diphosphate-Fuc pyrophosphorylase and human alpha1,3-fucosyltransferase 9 are combined in a Namalwa cellular model, the system can be applied to labeling of alkyne-modified fucosylated glycoproteins, e.g. for labeling of alkynyl fucose containing glycans, N-linked glycosylation site mapping and fucosylated glycoprotein identification by IGOT-LC-MS analysis, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacteroides fragilis
-
-
-
Bacteroides fragilis 9343
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-fucose
-
 Bacteroides fragilis ADP + beta-L-fucose 1-phosphate
-
 ?
ATP + L-fucose
-
 Bacteroides fragilis 9343 ADP + beta-L-fucose 1-phosphate
-
 ?
additional information development of an alternative way of labeling of fucosylated structures by metabolic engineering for the identification of fucosylated glycoproteins, using a chemoenzymatic approach. In this approach, the activities of Bacteroides fragilis 9343 L-fucokinase/guanosine-5'-diphosphate-Fuc pyrophosphorylase and human alpha1,3-fucosyltransferase 9 are combined in a Namalwa cellular model, the system can be applied to labeling of alkyne-modified fucosylated glycoproteins, e.g. for labeling of alkynyl fucose containing glycans, N-linked glycosylation site mapping and fucosylated glycoprotein identification by IGOT-LC-MS analysis, overview Bacteroides fragilis ?
-
 ?
additional information development of an alternative way of labeling of fucosylated structures by metabolic engineering for the identification of fucosylated glycoproteins, using a chemoenzymatic approach. In this approach, the activities of Bacteroides fragilis 9343 L-fucokinase/guanosine-5'-diphosphate-Fuc pyrophosphorylase and human alpha1,3-fucosyltransferase 9 are combined in a Namalwa cellular model, the system can be applied to labeling of alkyne-modified fucosylated glycoproteins, e.g. for labeling of alkynyl fucose containing glycans, N-linked glycosylation site mapping and fucosylated glycoprotein identification by IGOT-LC-MS analysis, overview Bacteroides fragilis 9343 ?
-
 ?

Synonyms

Synonyms Comment Organism
FKP
-
 Bacteroides fragilis
L-fucokinase/guanosine 5'-diphosphate-L-fucose pyrophosphorylase
-
 Bacteroides fragilis
L-fucokinase/guanosine-5'-diphosphate-Fuc pyrophosphorylase
-
 Bacteroides fragilis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Bacteroides fragilis

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the first step of the salvage pathway converting exogenous L-Fuc to GDP-L-Fuc Bacteroides fragilis
physiological function the bifunctional L-Fuc pyrophosphorylase, FKP, converts L-Fuc into GDP-L-Fuc via an L-Fuc-1-phosphate intermediate Bacteroides fragilis