Cloned (Comment) | Organism |
---|---|
expression as fusion protein with maltose-binding protein in Escherichia coli strain BL21 (DE3) | Vitis vinifera |
Crystallization (Comment) | Organism |
---|---|
purified apo-enzyme, mixing of 10 mg/ml protein in 10 mM Tris-HCl pH 7.5 with 9% w/v PEG 6K, 5% v/v ethylene glycol, 5% v/v glycerol, 25 mM HEPES pH 7.5, 2% tert-butanol, 1 mM MgCl2, 1mM MnCl2, 24°C, 20-30 days, X-ray diffraction structure determination and analysis at 2.6 A resolution | Vitis vinifera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
ATP | pH and temperature not specified in the publication | Vitis vinifera |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Vitis vinifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
118800 | - |
gel filtration | Vitis vinifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-fucose | Vitis vinifera | - |
ADP + beta-L-fucose 1-phosphate | - |
? | |
additional information | Vitis vinifera | fucokinase:GDP-fucose pyrophosphorylase is a dual activity enzyme metabolizing fucose and nucleotide-sugar | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vitis vinifera | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant maltose-binding protein fusion protein from Escherichia coli strain BL21(DE3) by amylose affinity chromatography, cleavage of the tag by Genease I protease, followed by guanosine affinity chromatography and ultrafiltration | Vitis vinifera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-fucose | - |
Vitis vinifera | ADP + beta-L-fucose 1-phosphate | - |
? | |
additional information | fucokinase:GDP-fucose pyrophosphorylase is a dual activity enzyme metabolizing fucose and nucleotide-sugar | Vitis vinifera | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 118800 | Vitis vinifera |
Synonyms | Comment | Organism |
---|---|---|
FKP | - |
Vitis vinifera |
fucokinase:GDP-fucose pyrophosphorylase | - |
Vitis vinifera |
L-fucokinase:GDP-fucose pyrophosphorylase | - |
Vitis vinifera |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
ATP | pH and temperature not specified in the publication | Vitis vinifera |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Vitis vinifera |