Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli with chaperones GroEL, GroES | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
G133A | mutation abolishes GDP-L-fucose diphosphorylase activity, hardly affects fucokinase activity | Arabidopsis thaliana |
G830A | mutation reduces fucokinase activity by more than 90%, while preserving the GDP-L-fucose diphosphorylase activity | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
ATP | pH 7.5, 35°C | Arabidopsis thaliana | |
1 | - |
L-fucose | pH 7.5, 35°C | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | or Mn2+, absolutely required for fucokinase activity | Arabidopsis thaliana | |
Mn2+ | or Mg2+, absolutely required for fucokinase activity | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
116350 | - |
x * 116350, calculated, x * 120000, SDS-PAGE | Arabidopsis thaliana |
120000 | - |
x * 116350, calculated, x * 120000, SDS-PAGE | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
bifunctional enzyme, L-fucokinase and GDP-L-fucose diphosphorylase activities | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | peptide sequence does not contain a secretory signal or transmembrane domain | Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
recombinant protein with His-tag and fused to thioredoxin, partial purification | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | expression in all tissues examined | Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
82.9 | - |
pH 7.5, 35°C | Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, 1 mM PMSF, 0.2 M NaCl, 50 mM Tris-HCl buffer, pH 7.5, stable for 1 month | Arabidopsis thaliana |
4°C, 1 day, loss of 50% of GDP-L-fucose diphosphorylase activity | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-fucose | - |
Arabidopsis thaliana | ADP + beta-L-fucose 1-phosphate | - |
? | |
additional information | enzyme generates GDP-L-fucose from L-fucose 1-phosphate and GTP. No substrates for fucokinase activity are: L-arabinose, glucose, galactose, mannose, xylose, L-rhamnose, galactosamine, glucosamine, N-acetylglucosamine | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 116350, calculated, x * 120000, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
Fkgp | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
optimum for fucokinase activity | Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
loss of 99% of activity | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
L-fucose | pH 7.5, 35°C | Arabidopsis thaliana | |
0.38 | - |
ATP | pH 7.5, 35°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.5 | - |
optimum for fucokinase activity | Arabidopsis thaliana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Arabidopsis thaliana | calculated | - |
5.9 |