Literature summary for 2.7.1.5 extracted from

Grueninger, D.; Schulz, G.E.
Structure and reaction mechanism of L-rhamnulose kinase from Escherichia coli (2006), J. Mol. Biol., 359, 787-797.

Search for more literature for 2.7.1.5

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain JM105	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop and sitting drop vapour diffusion methods using 17% (w/v) PEG 8000 and 120 mM LiCl	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
52000	-	gel filtration	Escherichia coli
54110	-	calculated from amino acid sequence	Escherichia coli

Organic Solvent Stability

Organic Solvent	Comment	Organism
2-mercaptoethanol	incubation over more than 5 h in fresh 20 mM 2-mercaptoethanol does not change the activity	Escherichia coli
dithiothreitol	incubation over more than 5 h in fresh 25 mM dithiothreitol does not change the activity	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P32171	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-Sepharose column chromatography and Superdex 200 gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-fructose	-	Escherichia coli	ADP + L-fructose 1-phosphate	-	?
ATP + L-rhamnulose	-	Escherichia coli	ADP + L-rhamnulose 1-phosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 52000, gel filtration	Escherichia coli

Synonyms

Synonyms	Comment	Organism
L-rhamnulose kinase	-	Escherichia coli
RhuK	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Escherichia coli

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Release 2023.1 (January 2023)