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Literature summary for 2.7.1.49 extracted from

  • Mizote, T.; Tsuda, M.; Smith, D.D.; Nakayama, H.; Nakazawa, T.
    Cloning and characterization of the thiD/J gene of Escherichia coli encoding a thiamin-synthesizing bifunctional enzyme, hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (1999), Microbiology, 145, 495-501.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli
Escherichia coli BL21(DE3) (pLysS) transformed with pTRD63 carrying ORF2, single gene thiD/J encodes 2 distinct enzyme activities, HMP kinase and HMP-P kinase, functional complemetation of Escherichia coli NI500, overexpression of ThiD Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27500
-
 SDS-PAGE Escherichia coli
31000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine Escherichia coli involved in thiamine biosynthetic pathway, first steps ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine Escherichia coli BL21(DE3) (pLysS) involved in thiamine biosynthetic pathway, first steps ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 BL21(DE3) (pLysS)
-
Escherichia coli P76422 nucleotide sequence of thiD
-
Escherichia coli BL21(DE3) (pLysS)
-
 BL21(DE3) (pLysS)
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.329
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine
-
 Escherichia coli ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine involved in thiamine biosynthetic pathway, first steps Escherichia coli ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine
-
 Escherichia coli BL21(DE3) (pLysS) ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine involved in thiamine biosynthetic pathway, first steps Escherichia coli BL21(DE3) (pLysS) ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
additional information bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase Escherichia coli ?
-
 ?
additional information bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase Escherichia coli BL21(DE3) (pLysS) ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 27500, SDS-PAGE Escherichia coli