Cloned (Comment) | Organism |
---|---|
gene UCK1, recombinant expression of N-terminally His-tagged isozyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
gene UCK2, recombinant expression of N-terminally His-tagged isozyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Homo sapiens | |
0.1 | - |
cytidine | recombinant isozyme UCK2, pH 7.6, 37°C | Homo sapiens | |
0.13 | - |
uridine | recombinant isozyme UCK2, pH 7.6, 37°C | Homo sapiens | |
4 | - |
cytidine | recombinant isozyme UCK1, pH 7.6, 37°C | Homo sapiens | |
5.1 | - |
uridine | recombinant isozyme UCK1, pH 7.6, 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cytidine | Homo sapiens | - |
ADP + CMP | - |
? | |
ATP + uridine | Homo sapiens | - |
ADP + UMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9BZX2 | - |
- |
Homo sapiens | Q9HA47 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged isozyme UCK1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Homo sapiens |
recombinant N-terminally His-tagged isozyme UCK2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
FISK cell | - |
Homo sapiens | - |
IMR-32 cell | - |
Homo sapiens | - |
NASS cell | - |
Homo sapiens | - |
neuroblastoma cell | generation of a specific antibody against UCK1 and analysis of expression of both UCK isoforms 1 and 2 in a panel of neuroblastoma cell lines, overview. No differences are observed for UCK1 and UCK2 expression between MYCN-single copy cell lines and MYCN-amplified neuroblastoma cell lines | Homo sapiens | - |
SH-SY5Y cell | - |
Homo sapiens | - |
SJNB-10 cell | - |
Homo sapiens | - |
SJNB-8 cell | - |
Homo sapiens | - |
SK-N-BE(2) cell | - |
Homo sapiens | - |
SK-N-SH cell | - |
Homo sapiens | - |
Tet21N cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cytidine | - |
Homo sapiens | ADP + CMP | - |
? | |
ATP + uridine | - |
Homo sapiens | ADP + UMP | - |
? | |
additional information | uridine-cytidine kinase (UCK) catalyzes the phosphorylation of uridine, cytidine, and several pyrimidine ribonucleoside analogues | Homo sapiens | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, recombinant isozyme UCK2, SDS-PAGE | Homo sapiens |
? | x * 31000, recombinant isozyme UCK1, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
UCK1 | - |
Homo sapiens |
UCK2 | - |
Homo sapiens |
uridine-cytidine kinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.59 | - |
uridine | recombinant isozyme UCK1, pH 7.6, 37°C | Homo sapiens | |
0.69 | - |
cytidine | recombinant isozyme UCK1, pH 7.6, 37°C | Homo sapiens | |
210 | - |
cytidine | recombinant isozyme UCK2, pH 7.6, 37°C | Homo sapiens | |
470 | - |
uridine | recombinant isozyme UCK2, pH 7.6, 37°C | Homo sapiens |